METALLOPROTEIN STRUCTURES BY PARALLEL FIELD EPR

RR等金属酶活性中心的表征 （核糖核苷酸还原酶）和MMO（甲烷单加氧酶）通过电子 如果完整，顺磁共振（EPR）可提供结构信息 可以得到EPR信号。 但部分EPR信号被发现为 金属蛋白中的许多高自旋金属离子和金属离子簇 和模型系统。 在这种情况下，Hendrich和 Debrunner，涉及测量两个区域中的部分EPR信号 正常模式和平行模式实验（振荡磁场） 给出了信号定量所需的信息， 金属蛋白位点，并最终用于测试结构模型。 该技术适用于几个蛋白质和模型系统的网站。 这种方法的惊人成功激发了一种重要的仪器 供应商Bruker为其EPR生产并联模式配件 系统. 这是一个持续多年的项目。 811-12

Characterization of active sites in metalloenzymes such as RR (ribonucleotide reductase) and MMO (methane monooxygenase) by electron paramagnetic resonance (EPR) is structurally informative if complete EPR signals can be obtained. But partial EPR signals are found for many high-spin metal ions and metal ion clusters in metalloproteins and model systems. In this case, the method of Hendrich and Debrunner, involving measurement of the partial EPR signals in both normal mode and parallel-mode experiments (oscillating magnetic field) gives the information necessary for quantitation of the signals from the metalloprotein sites and ultimately for testing structural models. This technique is applied to several protein and model system sites. The striking success of this method has stimulated a major instrument supplier, Bruker, to produce parallel-mode accessories for their EPR systems. This is a multi-year ongoing project. 811-12