SEAWATER SYSTEM RENOVATION FOR MARINE PHYSIOLOGY LAB

这个动物资源项目的长期目标是提高 沙漠山岛生物实验室(MDIBL)的能力 为研究人员提供海洋物种，用于基础生物医学和 毒物学研究。MDIBL是一家国际研究中心， 访问调查员利用独特的海洋物种进行调查 构成器官基本功能的分子机制，如 如肾、肝、脑、心、眼。MDIBL是 目前全年都有一项研究项目在发展中 生物学和一个企业实验室，正在合作的 鱼胚胎和转基因动物饲养设施的开发。 MDIBL能够为海洋动物，如鲨鱼，滑板， 比目鱼、海豚、剑鱼、螃蟹、海胆、沙鱼和蜗牛 完全依赖于海水系统的连续功能 它从法国海湾吸收海水，并将其分配到 单独的实验室和中央封存设施。当前的海水 制度是几十年逐步演变的结果，特别是 维修和改建。因此，当前的制度是高度 不充分的抽水和次优的分配以及 故障率高且不易老化的部件 维护好了。该系统的频繁故障导致样品丢失 或在次优条件下进行维护，从而削弱了 实验室为研究提供动物。具体目标是1)升级 安装大容量自吸泵的海水进水系统 配有重复的进气管路，以实现周期性预防 维护；以及提高抽水的安全性和可及性 说明；2)彻底重新设计和改造海水分布 确保到所有实验室和实验室的足够流量的系统 区域，并允许重复的线条，便于清洁和防止 冬季冻结鱼线；3)翻新养鱼区，以 增加海水的容量和质量控制，并允许 扩大季节性和全年使用；以及4)实施扩大的 动物健康计划，将包括增加对动物的监测 饲养动物所需的制革和兽医监督 周期很长。拟议的翻修将增强MDIBL的能力 向来访的调查人员提供海洋标本，允许延长一年- 充分利用动物设施，为 重建用于产生和维护转基因的核心设施 老鼠。

The long range goal of this animal resource project is to enhance the ability of the Mount Desert Island Biological Laboratory (MDIBL) to provide marine species to investigators for use in basic biomedical and toxicological research. MDIBL is an international research center where visiting investigators make use of unique marine species to investigate the molecular mechanisms that underlie the basic function of organs such as the kidney, the liver, the brain, the heart and the ye. MDIBL is currently the year round home for one research program in Developmental Biology and a corporate laboratory, that is collaborating in the development of facility for rearing fish embryos and transgenic animals. The ability of MDIBL to provide marine animals such as the shark, skate, flounder, sculpin, killifish, crabs, sea urchins, sand dollars, and snails is entirely dependent on the continuous function of a seawater system which takes in seawater from Frenchman Bay and distributes it to individual laboratories and central hold facilities. The current seawater system is the result of several decades of gradual evolution, ad hoc repairs and alterations. As a consequence the current system is highly compromised by inadequate pumping and suboptimal distribution as well as aging components that have a high failure rate and cannot be easily maintained. Frequent failure of the system leads to the loss of specimens or maintenance in suboptimal conditions thus impairing the ability of the Lab to supply animals for research. The specific aims are 1) to upgrade the seawater intake system by installing high capacity, self priming pumps equipped with duplicate intake lines to permit periodic preventative maintenance; and to improve the safety and accessibility of the pumping stating; 2) to completely redesign and renovate the seawater distribution system to assure adequate flow rates to all laboratories and holding areas, and allow for duplicate lines that facilitate cleaning and prevent freezing of the lines in winter; 3) to renovate fish holding areas to increase the capacity and the quality control of the seawater and permit expanded seasonal and year-round use; and 4) to implement an expanded animal health program that will include increased monitoring of animal holding tans and veterinary supervision necessary for holding animals for long periods. The proposed renovation will enhance the ability of MDIBL to provide marine specimens to visiting investigators, permit expanded year- round use of the animal facility and provide the foundation for reestablishing a core facility for generating and maintaining transgenic mice.