PRIMATE MODEL OF DENDRITIC CELL-BASED IMMUNOTHERAPY

描述(改编自申请人的摘要)：的总体目标 本次SERCA申请是为了在 免疫学领域，最终目标是发展职业生涯 比较医学领域的独立研究员。应聘者承诺 使用他的技能来研究人类疾病的动物模型。这项研究 该提案的核心是基本假设，即疫苗的免疫原性 可以通过将该抗原呈现在 强大的免疫刺激树突状细胞。新世纪头三年 大奖将重点检验这一假说，使用与肿瘤相关的 抗原，上皮细胞粘蛋白，已被确定为 乳腺癌、胰腺癌患者肿瘤特异性T淋巴细胞的检测 和卵巢癌。该应用程序建议使用一种新颖且高度 相关的灵长类动物模型，即黑猩猩，候选人从该模型中 开发了培养树突状细胞用于体外操作的技术。 在最后两年，重点将转向检验这一假说。 在黑猩猩模型中使用HIV包膜糖蛋白。这些 研究将把第一次研究的经验与 候选人在病毒免疫学和病毒学方面的背景。导师， 奥利维拉·J·芬恩教授在人类肿瘤免疫学领域获得资助， 该大学还有其他几个研究项目，研究 癌症的免疫疗法。这位候选人获得了另外三位候选人的支持 医学院的教职员工，包括罗纳德·C教授。 Montelaro是一位慢病毒研究人员，他在独立报担任顾问 第四年和第五年的学习。

DESCRIPTION (Adapted from the applicant's abstract): The broad objective of this SERCA application is to obtain in-depth research experience in the field of immunology, with the ultimate goal being to develop a career as an independent researcher in comparative medicine. The candidate is committed to using his skills to study animal models of human disease. The research proposal centers around the basic hypothesis that the immunogenicity of an antigen can be augmented by presenting that antigen in the context of the potent immuno-stimulatory dendritic cell. The first three years of the award will focus on testing this hypothesis using the tumor-associated antigen, epithelial cell mucin, which has been identified as a target for tumor-specific T lymphocytes of patients suffering from breast, pancreatic, and ovarian cancers. The application proposes use of a novel and highly relevant primate model, the chimpanzee, from which the candidate has developed techniques to cultivate dendritic cells for manipulation in vitro. In the final two years the emphasis will switch to testing this hypothesis using the envelope glycoprotein of HIV in the chimpanzee model. These studies will incorporate the experience from the first studies with the candidate's background in viral immunology and virology. The mentor, Professor Olivera J. Finn, is funded in the field of human tumor immunology, and the university has several other research programs studying the immunotherapy of cancer. The candidate has the support of three additional faculty members from the School of Medicine, including Professor Ronald C. Montelaro, a lentivirus researcher who act as an advisor in the independent studies of Years 4 and 5.