ELF ELECTROMAGNETIC FIELDS AND CANCER

描述：流行病学研究与各种极端 低频（ELF）电磁场与白血病、脑和乳腺 癌 不幸的是，一个合理的科学机制， 癌症是未知的。 因此，一个长期目标是建立一个 ELF场和癌症之间的机械联系。 最近，我们展示了 人类细胞的代谢振荡可以在迁移过程中产生共振， 在存在相位匹配的ELF电场的情况下， 环境相关强度区域。 环境电磁 与代谢振荡频率相匹配的次谐波可以诱导 代谢共振，从而极大地改变正常的振荡 代谢反应，并因此，细胞形态和功能（细胞 迁移/侵入/粘附特性）。 为了测试代谢共振 提供了ELF场和癌症之间的机械联系，我们将揭露 肿瘤细胞，白血病细胞，和控制电场， 迁移、粘附和侵入活动（细胞分离模型， 侵袭和转移）。 为了控制代谢阶段 振荡，我们将监测NAD（P）H自发荧光（共振）， 相位不匹配的条件。 我们将描述电场的特征 代谢共振强度依赖性。 我们还将检查细胞 形态学、细胞骨架变化和细胞周围蛋白水解 代谢共振 我们的机电耦合假设可能是一个 弱场与代谢共振的物理机制 下游生物效应 为了测试这种主要的耦合机制，我们将测试 细胞表面电荷在代谢共振中的作用。 初步数据 表明高振幅NAD（P）H振荡与 超氧阴离子产生。 我们将研究电场强度， 白细胞内活性氧代谢产物产生的阶段依赖性， 肿瘤和白血病细胞。 利用彗星和末端脱氧核苷酸 转移酶测定，我们将代谢共振/氧化应激与DNA 损害 一系列机械事件将极低频场与 癌细胞的粘附/运动/侵袭特性、代谢和DNA 将识别损伤;这可能为ELF提供物理化学基础 健康影响。

DESCRIPTION: Epidemiological studies have variously associated extremely low frequency (ELF) electromagnetic fields with leukemia, brain and breast cancer. Unfortunately, a rational scientific mechanism linking such fields with cancer is not known. Thus, one long-range goal is to establish a mechanistic link between ELF fields and cancer. Recently, we have shown the metabolic oscillations of human cells can resonate during migration or adherence in the presence of phase-matched ELF electric fields within the environmentally relevant intensity region. Environmental electromagnetic subharmonics that match metabolic oscillation frequencies could induce metabolic resonance thereby dramatically altering normal oscillatory metabolic reactions and, consequently, cell morphology and function (cell migration/invasive/adherence properties). To test if metabolic resonance provides a mechanistic link betwee ELF fields and cancer, we will expose tumor cells, leukemic cells, and control to electric fields during migration, adherence and invasive activities (models of cell detachment, invasion and metastasis). To control for the phase of metabolic oscillations, we will monitor NAD (P) H autofluorescence in (resonance) and phase-mismatched conditions. We will characterize the electric field intensity dependence of metabolic resonance. We will also examine cell morphology, cytoskeletal changes and pericellular proteolysis during metabolic resonance. Our electromechanical coupling hypothesis may be a physical mechanism linking weak fields to metabolic resonance and its downstream bioeffects. To test this primary coupling mechanism we will test the role of cell surface charges in metabolic resonance. Preliminary data show that high-amplitude NAD (P) H oscillations are associated with superoxide anion production. We will study the electric field intensity and phase-dependence of reactive oxygen metabolite production within leukocytes, tumor and leukemic cells. Using the comet and terminal deoxynucleotidyl transferase assays, we will link metabolic resonance/oxidant stress with DNA damage. A mechanistic series of events linking ELF fields with adherence/locomotion/invasive properties of cancer cells, metabolism and DNA damage will be identified; this may provide a physicochemical basis for ELF health effects.