Cell Cycle Regulation By the AML1B Transcription Factor

描述(由申请人提供) AML-1B基因产物与许多易位有关，导致 为了白血病。AML-1B是一种转录因子，被证明参与了 造血分化。我们的初步结果显示， 表达野生型AML-1B可缩短细胞周期的G1期。目标 本研究的重点是确定AML-1B影响细胞的途径(S) 循环递增。我们将使用DNA微阵列技术来询问 AML-1B的转录靶点，靶向什么途径，以及 哪些靶点与观察到的AML-1B细胞周期表型有关。我们 将利用AML-1b属于一个家族的事实 所有转录因子都与相同的增强子基序结合。然而，在这件事上 家族中，只有AML-1B影响细胞周期进程。因此，通过比较 AML-1B及其家族成员之一(AML-3)WE的转录靶点 应该能够消除许多不太可能涉及的目标 在细胞周期表型上。这应该会增加 成功识别影响细胞周期进程的AML-1B靶点。 从而带头鉴定第一次转录是如何 缩短细胞周期G1期的因子完成了这一壮举。

DESCRIPTION (provided by applicant) The AML-1B gene product has been implicated in numerous translocations leading to leukemia. AML-1B is a transcription factor shown to be involved in hematopoietic differentiation. Our preliminary results show that over expressing wild-type AML-1B shortens the G1 phase of the cell cycle. The goals of this research are to identify the pathway(s) by which AML-1B affects cell cycle progression. We will use DNA microarray technology to ask what are the transcriptional targets of AML-1B, what pathways the targets feed into, and which targets are involved in the cell cycle phenotype observed for AML-1B. We will take advantage of the fact that AML-lB belongs to a family of transcription factors that all bind to the same enhancer motif. Yet out of this family, only AML-1B affects cell cycle progression. Therefore, by comparing transcriptional targets of AML-1B with one of its family members (AML-3) we should be able to eliminate numerous targets that are not likely to be involved in the cell cycle phenotype. This should increase the probability of successfully identifying targets of AML-1B which affect cell cycle progression. Thus leading the way for the identification of how the first transcription factor shown to shorten the G1 phase of the cell cycle accomplishes this feat.