STUDIES OF THE V-<I>ETS</I> ETS1 AND FLI1 TRANSCRIPTION FACTORS IN HEMATOPOIETIC

ETS转录因子家族已被证明与细胞增殖和多种肿瘤转化事件有关，特别是那些涉及红系谱系的肿瘤转化事件。我们一直对ETS转录因子家族的两个成员ETS-1和fl -1基因在造血肿瘤和正常分化中的作用感兴趣。ETS家族的成员已被证明参与红细胞和巨核细胞在体内的分化。我们之前的研究表明，FLI-1在某些多能人造血细胞系中发挥积极作用，激活巨核细胞分化过程中正常表达的基因，同时阻断红细胞分化相关基因的表达。我们进一步研究了MKK/MAPK和fl -1在K562和HEL细胞中激活巨核细胞标记物表达的机制。我们的数据表明，这些影响可能涉及两个独立的途径，外源FLI-1的存在改变了MKK/MAPK的激活和抑制作用。此外，这些研究表明，gpII_B和gpIII_A蛋白水平的调节可能受到这些途径的不同影响。-红细胞，ETS1, FLI1， HEL，造血，K, 2，巨核细胞，转录因子，-既不是人类受试者也不是人类组织

The ETS family of transcription factors have been shown to be associated cell proliferation and a variety on neoplastic transforming events, especially those involving the erythroid lineage. We have been interested in the role of the ETS-1 and FLI-1 genes, two members of the ETS family of transcription factors, in hematopoeitic neoplasia and normal differentiation. Members of the ETS family have been shown to be involved in aspects of both erythroid and megakaryocytic differentiation in vivo. Our previous work had indicated that FLI-1 acted positively in certain pluripotent human hematopoeitic cell lines to activate genes normally expressed during megakaryocytic differentiaton, while blocking expression of genes associated with erythroid differentiation. We have extended our studies of the mechanisms by which MKK/MAPK and FLI-1 both can activate the expression of megakaryocytic markers in K562 and HEL cells. Our data indicate that these effects may involve two separate pathways, and that the presence of exogenous FLI-1 alters the effects of MKK/MAPK activation and inhibition. In addition these studies suggest that the regulation of the gpII_B and gpIII_A protein levels may be differentially affected by these pathways. - erythroid, ETS1, FLI1, HEL, hematopoiesis, K, 2, megakaryocyte, transcription factor, - Neither Human Subjects nor Human Tissues