MOLECULAR DIAGNOSIS OF FELINE INFECTIOUS PERITONITIS VS ENTERIC CORONA VIRUS

建立了用逆转录聚合酶链式反应(RT-PCR)检测猫传染性腹膜炎(FIP)病毒RNA的方法，并与猫肠道冠状病毒(FECV)RNA进行了鉴别。由于FECV的突变被认为是FIP病毒的来源，这项技术对于单独表明最致命的FIP病毒的存在是重要的。它还可以为兽医提供潜在的FIP爆发的迹象，以及一种从NIH调查人员依赖的猫科动物中消除病毒的方法。许多样本已经使用这一方案进行了处理，以帮助国立卫生研究院的设施根除这种病原体，并评估这一方法在流行病学研究中的效果。-FIP、FECV、RT-PCR、RNA、猫、病毒、暴发

A protocol has been developed using RT-PCR (reverse transcriptase polymerase chain reaction) with nested synthetic oligomers to detect Feline Infectious Peritonitis (FIP) viral RNA and to distinguish it from Feline Enteric Corona Virus (FECV) RNA. Since it is thought that a mutation in FECV is the source of the FIP virus, this technique is important to indicate the presence of the mostly fatal FIP virus alone. It can also give the veterinarian an indication of a potential FIP outbreak and a method for eliminating the virus from catteries relied on by NIH investigators. Many samples have been processed using this protocol to assist NIH facilities in eradication of this pathogen and for evaluating the efficacy of this methodology for epidemiological studies. - FIP, FECV, RT-PCR, RNA, feline, viral, outbreak