METHODS OF IONIZATION IN MASS SPECTROSCOPY

分析蛋白质结构的方法正在改进，并在独立和合作研究项目中得到应用和测试。对于吲哚胺-2，3-双加氧酶作为模型糖蛋白的一级结构测定，在线和基于凝胶的层析系统正在进行测试。识别翻译后修饰或独特基因产物的合作研究正在进行中。还在设计和测试方法，以提高使用质谱仪检测痕量有机化合物的能力。为提高负电离质谱检测的电子俘获性能，新的衍生化试剂和方法的开发一直在继续，并应用于细胞培养中谷氨酸的分析。作为~(13)C-色氨酸代谢产物的~(13)C标记NAD和~(13)C-NADP的定量分析方法还在继续发展。全13C和15N标记的NAD已从酵母中分离出来，用作分析标准。亲水作用层析已成功地用于改进核苷和NAD的分离。设计了一种毛细管区带电泳和电喷雾质谱仪联用的接口，以增强对从细胞提取液和组织中分离出来的多肽和混合物的检测。-液相色谱、质谱学、电喷雾、毛细管电泳法、衍生化、MALDI/TOF、蛋白质结构

Methods for the analysis of protein structures are being improved, applied and tested in both independent and collaborative research projects. Chromatographic systems, both on-line and gel-based are being tested with for the primary structure determination of indoleamine-2,3- dioxygenase as a model glycoprotein. Collaborative studies on the identification of post-translational modifications or unique gene products are in progress. Methods are also being devised and tested to improve the detectability of trace organic compounds using mass spectrometry. The development of new derivatization reagents and methods to impart improved electron capture properties for negative ionization mass spectrometric detection has been continued and applied to the analysis of glutamate in cell cultures. Liquid-chromatography - electrospray mass spectrometry methods continue to be developed for the quantitative analyses of 13C-labeled NAD and NADP as products of 13C- tryptophan metabolism. Fully 13C and 15N labeled NAD has been isolated from yeast for use as an analytical standard. Hydrophilic interaction chromatography has been successfully used for improved separations of nucleosides and NAD. An interface for coupling capillary zone electrophoresis and electrospray mass spectrometry has been designed to enable enhanced detection of peptides and mixtures isolated from cell extracts and tissues. - liquid chromatography, mass spectrometry, electrospray, capillary electrophoresis, derivatization, MALDI/TOF, protein structure