Multidimensional Gas Chromatograph for Toxicological An*

描述(由申请人提供)： 本项目将探索双二元柱的应用 全二维气相色谱(GC X 2GC)用于表征和 对有毒化学物质的暴露进行量化。GC x 2GC提供高分辨率， 灵敏度高，分析时间短，只需一小部分天然气成本 色谱/质谱学技术。在第一阶段，(A)一个正常工作的GC x 2GC原型将被构建和表征，(B)保留数据库 将被开发以允许在广泛的范围内识别峰 层析条件，以及(C)挥发性有机化合物分布 吸烟者和不吸烟者的人体呼气样本将使用 GC x 2GC系统。GC x 2GC的高速和高分辨率 仪器允许呼吸样本在几个方面进行全面表征 几分钟。 在第二阶段的研究中，研究人员将(A)使用已建立的动物 确定呼气的GC x 2GC分析是否可以检测器官特异性的模型 毒性；(B)使用神经/模糊算法搜索化合物或模式 表明接触的化合物；和(C)表征其特异性和 特定化合物的敏感性因此被确定为毒性的标记物。 建议的商业应用： 虽然已经证明呼气分析可以用来表征对毒物的暴露和反应，但传统的GC/MS方法非常昂贵和耗时。正在评估的新型GC x 2GC仪器相对低成本、高速和高分辨率，使呼气样本在几分钟内就能得到全面的表征。一旦完全开发，这种仪器和使用它的化验服务预计将有一个巨大的市场。这一市场将包括对暴露地点空气的实时分析，以及对暴露个人的并行监测和跟踪。

DESCRIPTION (provided by applicant): This project will explore the application of a dual-secondary column comprehensive two-dimensional gas chromatograph (GC x 2GC) to characterize and quantitate exposure to toxic chemicals. GC x 2GC provides high resolution, high sensitivity, and short analysis times for a fraction of the cost of gas chromatography/mass spectrometry techniques. In Phase I, (a) a working GC x 2GC prototype will be constructed and characterized, (b) a retention database will be developed to allow peaks to be identified under a wide range of chromatographic conditions, and (c) volatile organic compound profiles of human breath samples from smokers and nonsmokers will be analyzed using the GC x 2GC system. The high-speed and high-resolution of the GC x 2GC instrument allows breath samples to be comprehensively characterized in a few minutes. In Phase II studies, the investigators will (a) employ established animal models to determine if GC x 2GC analysis of breath can detect organ-specific toxicity; (b) use neural/fuzzy algorithms to search for compounds or patterns of compounds indicative of exposure; and (c) characterize the specificity and sensitivity of specific compounds thus identified as markers of toxicity. PROPOSED COMMERCIAL APPLICATION: Although it has been demonstrated that breath analysis can be used to characterize exposure and reaction to toxicants, conventional GC/MS methodology is very expensive and time-consuming. The relatively low cost, high-speed and high-resolution of the novel GC x 2GC instrument under evaluation allows breath samples to be comprehensively characterized in a few minutes. Once fully developed, there is expected to be a significant market for this instrument and for assay services that employ it. This market will include real time ananlysis of air in sites exposure, and parallel monitoring and tracking of exposed individuals.