IMPROVED CLASSIFIERS FOR AUTOMATED MULTIPLEX FISH

描述（摘要，来自应用程序）：自动核型分析是一种 细胞遗传学实验室的重要程序。多重荧光 原位杂交（M-FISH）是一种最新的发展， 染色体涂染探针和多光谱图像分析来识别细微的 和复杂的染色体重排。它承诺使自动化 核型分析更快，更准确，更容易解释，无论是在临床 情况和癌症研究。限制能力的主要因素 M-FISH用于解析异常染色体中DNA的染色体起源， 像素分类精度该项目的目标是开发改进的 M-FISH的软件技术，以显着提高像素的准确性 分类在M-FISH系统，但最大限度地发挥这一重要的影响， 细胞遗传学实践的新技术。这些工具 商业上可获得的现在仅实现基本的像素分类 用于识别DNA同源起源的算法。本课题 将最先进的模式识别技术应用于M-FISH， 像素分类精度远远超过目前商业 系统.改进后的M-FISH系统将自动找到 并标记微妙和复杂的结构异常（插入和 遗传物质的易位）有助于癌症研究和遗传 诊断.一旦我们在第一阶段确定了可行性，我们将使用第二阶段。 二是充分发挥系统的优势，在临床上进行系统的测试 用于外周血管穿刺术的产前、产后和癌症样本 血液和骨髓这些增强功能将集成到PSI的M-FISH中 仪器，目前是细胞遗传学自动化PowerGene系列的一部分 产品.由此产生的商业仪器将优于目前的上级 用于阐明染色体内结构重排的现有系统。 拟定商业应用： 一旦新技术被开发出来并能用于常规应用， 将被纳入PSI的细胞遗传学自动化设备的PowerGene产品线， 无论是在销售的新系统中，还是作为对已在使用的现有系统的升级， 细胞遗传学实验室 新开发的染色体绘画探针将被添加到PSI的 试剂生产线，从而使该技术快速商业化。

DESCRIPTION (Abstract, from the application): Automated karyotyping is an important procedure in cytogenetics labs worldwide. Multiplex fluorescence in situ hybridization (M-FISH) is a recent development that uses multicolor chromosome painting probes and multispectral image analysis to identify subtle and complex chromosomal rearrangements. It promises to make automated karyotyping faster, more accurate, and easier to interpret both in clinical situation and in cancer research. The major factor limiting the ability of M-FISH to resolve the chromosomal origin of the DNA in abnormal chromosomes is pixel classification accuracy. The goal of this project to develop improved software techniques for M-FISH to improve significantly the accuracy of pixel classification in M-FISH systems, but maximizing the impact of this important new technology on the practice of cytogenetics. The instruments that are commercially available now implement only rudimentary pixel classification algorithms for identifying the homologue origin of DNA. In this project, we will apply state-of-the-art pattern recognition techniques to M-FISH to improve pixel classification accuracy far beyond what is current offered by commercial systems. The improved M-FISH system that will result will automatically find and flag both subtle and complex structural abnormalities (insertions and translocations of genetic material) assisting both cancer research and genetic diagnosis. Once we have established feasibility in Phase I, we will use Phase II to develop the improvements fully and test the system in routine clinical use on prenatal, postnatal and cancer specimens from amniocentesis, peripheral blood, and bone marrow. The enhancements will be integrated into PSI's M- FISH instrument, currently part of the PowerGene line of cytogenetics automation products. The resulting commercial instrument will be superior to currently available systems for elucidating structural rearrangements within chromosomes. PROPOSED COMMERCIAL APPLICATION: As soon as the new techniques are developed and qualified for routine application, they will be incorporated into PSI's PowerGene product line of cytogenetics automation equipment, both in new systems sold and as an upgrade to existing systems already in use in cytogenetics labs. Newly developed chromosome painting probes will be added to PSI's reagent product line, thus commercializing the technology quickly.