Selective Inhibitors of Pancreatic Cholesterol Esterase

描述(摘自申请者摘要)：本申请的具体目的 酯酶(CE)抑制剂在急性胆固醇吸收试验中的应用。仓鼠会 BE用20-200种CE-抑制剂灌胃~3H标记的胆固醇 伞形花环。24小时后，肝脏和血清胆固醇吸收将 接受化验。肝脏和血清胆固醇水平将在 亚慢性仓鼠试验。饲料中添加20-200微克分子的Ce-抑制剂 补充0.2%的胆固醇和0.1%的胆酸 几天。将测定肝脏和血清中的胆固醇。在第三项研究中， 毒性将在CE抑制剂的30天喂养试验后确定。 前两次实验中确定的CE-抑制剂的最佳剂量和 在食物中添加200微克，添加1%的胆固醇和0.5%的胆固醇 %的胆酸。临床化学，肝脏和血清胆固醇， 将进行尿液分析和组织病理学检查。

DESCRIPTION (Scanned from the Applicant's Abstract): The specific aim of this esterase (CE) inhibitor in an acute cholesterol absorption assay. Hamsters will be gavage fed 3H-labeled cholesterol with CE-inhibitor ranging from 20-200 umoles. Twenty-four hours later, hepatic and serum cholesterol absorption will be assayed. Hepatic and serum cholesterol levels will be determined in a subchronic hamster assay. CE-inhibitor from 20-200 umoles will be fed in chow supplemented with 0.2 percent cholesterol and 0.1 percent cholic acid for 4 days. Hepatic and serum cholesterol will be determined. In a third study, toxicity will be determined following a 30 day feeding trial of CE inhibitor. The optimum dose of CE-inhibitor determined in the previous two experiments and 200 umoles will be added to chow supplemented with 1 percent cholesterol and 0.5 percent cholic acid. Clinical chemistry, hepatic and serum cholesterol, urinalysis and histopathology will be obtained.