SCIENTIFIC CORE

科学核心旨在作为成像的中心资源 应用于大鼠脑和三叉神经节切片的技术， 豚鼠，以及死后的人体组织。 第一个项目将使用受体放射自显影检测5-HT 1D样 和GABA受体结合位点在三叉神经系统的啮齿动物， 死后人脑，以及原位杂交检测 对应的信使RNA。 第二个项目将使用[14 C]2-脱氧葡萄糖放射自显影技术 测量局部葡萄糖利用率作为神经元 在疼痛的硬脑膜刺激后大鼠大脑中的激活。 神经元 还将在相同的动物中评估激活（在连续的脑中 通过检测早期基因c-fos的信使RNA， 原位杂交 使用相同的刺激，我们将使用 在另一组动物中进行[14 C]碘安替比林放射自显影， 有害脑膜刺激对代谢/血流的影响 偶合器. 这些实验的结果可能会影响对 功能激活研究作为最后一个项目的目标。

The Scientific Core is intended to serve as a central resource for imaging techniques applied to brain and trigeminal ganglion sections from rats and guinea pigs, as well as from post mortem human tissue. The first project will use receptor autoradiography to detect 5-HT 1D-like and GABA receptor binding sites ina the trigeminal system of rodents and post mortem human brain, as well as in situ hybridization to detect the corresponding messenger RNA's. The second project will use [14C]2-deoxyglucose autoradiography technique to measure regional glucose utilization as an indicator of neuronal activation in the rat brain after painful dural stimulation. Neuronal activation will also be assessed in the same animals (in consecutive brain sections) by detecting the messenger RNA of the early gene c-fos using in situ hybridization. Using the same stimulus, we will use [14C]iodoantipyrine autoradiography in another set of animals to determine the consequences of noxious meningeal stimulation on metabolism/blood flow coupling. The results of such experiments may impact on the interpretation of functional activation studies as an aim of the last project.