INTRA SUBJECT VARIABILITY IN METABOLISM OF GLUCOSINOLATES

确定肠道菌群是否对硫代葡萄糖苷有贡献 在人类的新陈代谢中，我们评估了标准的术前效果 肠道准备对二硫代氨基甲酸摄入后排泄的影响 成熟西兰花中的硫代葡萄糖苷。准备硫代葡萄糖苷、小花 从冰包装的西兰花切开并立即在Stearn中煮熟- 夹套水壶(8 L水，5分钟)。小花被移走了， 剩余的液体减少到原来体积的大约一半(超过5 Min)，并重新加入煮熟的小花中，混合物 在瓦林搅拌机中均质化。抽取样本进行分析，并 匀浆储存在零下80摄氏度。 每182毫升中加入112克鲜重西兰花，含100微克非吲哚 硫代葡萄糖苷、0.5umol异硫氰酸酯和不残留黑芥子酶 活性；葡萄糖萝卜素为76。1%的硫代葡萄糖苷。

To determine whether enteric flora contribute to glucosinolate metabolism in humans, we evaluated the effect of a standard preoperative bowel preparation on dithiocarbainate excretion after ingestion of glucosinolates from mature broccoli. To prepare glucosinolates, florets from ice-packed broccoli were cut and immediately boiled in a stearn- jacketed kettle (8 L water, 5 min). Florets were removed, the remaining liquid was reduced to about half its original volume (over 5 min) and was added back to the cooked florets, and the mixture was homogenized in a Waring Blender. Samples were taken for analysis and the homogenate was stored at -80 degrees C. Individual doses consisted of 112 g fresh weight broccoli in 182 mL, containing 100umol non-indole glucosinolates, 0.5 umol isothiocyanates and no residual myrosinase activity; glucoraphanin constituted 76. 1 % of the glucosinolates.