GLUCOSINOLATE--ISOTHIOCYNATE CONVERSION AFTER EATING BROCCLI SPROUT HOMOGENATE

确定肠道植物群是否有助于硫苷 在人体代谢中，我们评估了标准术前 摄入二硫代氨基甲酸盐后的肠道准备 成熟花椰菜中的芥子油苷。 为了制备硫代葡萄糖苷，小花 从冰包装的西兰花被削减，并立即煮在一个stearn- 夹套釜（8L水，5分钟）。 小花被移走， 剩余的液体减少到其原始体积的约一半（超过5 min），并加回到煮过的小花中，并将该混合物浓缩。 在Waring Blender中均质化。 取样进行分析， 将匀浆储存在-80 ℃。 个体剂量包括 112 g鲜重西兰花在182 mL中，含有100 umol非吲哚 芥子油苷，0.5 μ mol异硫氰酸酯，无黑芥子酶残留 活性;萝卜硫苷构成76. 1%的硫代葡萄糖苷。

To determine whether enteric flora contribute to glucosinolate metabolism in humans, we evaluated the effect of a standard preoperative bowel preparation on dithiocarbainate excretion after ingestion of glucosinolates from mature broccoli. To prepare glucosinolates, florets from ice-packed broccoli were cut and immediately boiled in a stearn- jacketed kettle (8 L water, 5 min). Florets were removed, the remaining liquid was reduced to about half its original volume (over 5 min) and was added back to the cooked florets, and the mixture was homogenized in a Waring Blender. Samples were taken for analysis and the homogenate was stored at -80 degrees C. Individual doses consisted of 112 g fresh weight broccoli in 182 mL, containing 100 umol non-indole glucosinolates, 0.5) umol isothiocyanates and no residual myrosinase activity; glucoraphanin constituted 76. 1 % of the glucosinolates.