Core--Tissue Culture Core

组织培养核心将支持项目1、项目3和项目4的组织培养活动。(项目2将使用大鼠肝脏，项目5将使用完整的转基因动物)。这一核心将优化利用在过去18年中在该部门积累的广泛的组织培养专业知识。它将提供一个中央设施，用于获取和测试组织培养所需材料的质量，以供本计划项目的研究人员使用。这些材料将包括胎牛血清(FBS)，它经过一致性测试，然后订购足够大的数量，以便一批FBS将支持该计划项目的活动至少一年。新的组织培养塑料器皿在被更广泛地应用于计划项目实验室之前，也将由核心进行问题测试。核心将提供启动培养，并在要求时，将准备特定实验的培养。每一次培养都要向研究人员收费；这笔费用是为了鼓励优化使用来自核心的组织培养。细胞将通过精心的方法，使用标准化的方案在核心进行培养。针对成纤维细胞的内部方案现已发表(附录)。这些程序提供了培养中生物年龄(CPDL)的成纤维细胞培养的高度标准化，以及对组织培养的其他方面的严格控制，如支原体检测。包括PC12细胞在内的肿瘤细胞将通过我们在这个实验室已经标准化的技术进行培养。必要时，该核心还将准备富含神经元或神经胶质的原代培养物。核心的经验将有助于在任何项目中使用其他类型的单元。组织培养核心旨在增强特定研究人员实验室中的组织培养设施。它将为个体研究人员的组织培养需求提供最佳支持，同时让每个研究人员完全控制他们调查所需的培养物。

The Tissue Culture Core will support tissue culture activities in Project 1, Project 3, and Project 4. (Project 2 will use rat liver, and Project 5 will use intact transgenic animals). This Core will optimize the use of the extensive tissue culture expertise that has accumulated in this unit over the last 18 years. It will provide a central facility for obtaining and testing the quality of materials required for tissue culture by the investigators in this Program Project. The materials will include Fetal Bovine Serum (FBS), which is tested for consistency and then ordered in large enough amounts so that a single lot of FBS will support the activities of the Program Project for at least one year. New tissue culture plasticware will also be tested for problems by the Core before being used more widely within the Program Project laboratories. The Core will provide started cultures, and when requested, will prepare cultures for specific experiments. There will be a charge to the investigators for each culture; this fee is designed to encourage optimal use of tissue cultures from the core. Cells will be grown in the core by meticulous methods using well- standardized protocols. Inside the protocol for the fibroblasts has now been published (APPENDIX). These procedures provide a high degree of standardization of fibroblast cultures by biological age in culture (CPDL), as well as meticulous control of other aspects of tissue culture such as mycoplasma testing. Tumor cells, including PC12 cells, will be grown by techniques that we have already standardized in this laboratory. Where necessary, this core will also prepare primary neuron-enriched or glial- enriched cultures. The experience of the core will facilitate the use of other cell types for any of the PROJECTS. The Tissue Culture Core is designed to augment tissue culture facilities in the laboratories of the specific investigators. it will provide optimal support for the tissue culture needs of the individual investigators, while leaving each investigator in full control of the cultures needed for their investigation.