CORE--TREPONEMAL STRAINS LABORATORY

该中心将作为一个中心实验室，用于1）所有项目的梅毒螺旋体菌株的繁殖; 2）针对单个T。pallidum菌株和重组msp同源分子，用于项目1和2，用于用重组msp同源分子免疫兔子，用于项目3中的保护研究。T.苍白球不能在体外繁殖，必须在兔子中连续传代，需要专门的技术，特别是对于非Nichols菌株，其更难以生长。这些任务是相当耗时的，需要大量的技术人员的努力，膨胀作为一个大量的兔子。三个项目中提出的各种实验所需的密螺旋体可以更经济地提供，如果它们集中繁殖，就最大限度地减少兔子的数量以及技术人员的时间而言。向项目负责人提供由核心抗体产生的抗血清也将确保特异性的一致性，从而允许对不同项目的结果进行比较。核心将由Sheila Lukehart博士指导，她在致病性密螺旋体的传播方面有25年的经验。

This core will serve as a centralized laboratory for the 1) propagation of Treponema pallidum strains for all projects; 2) for production of antisera directed against individual T. pallidum strains and recombinant msp- homologue molecules for Projects 1 and 2 for immunization of rabbits with recombinant msp-homologue molecules for protection studies in Project 3. T. pallidum cannot be propagated in vitro and must be continually passaged in rabbits, requiring specialized expertise, particularly for the non-Nichols strains, which are much more difficult to grow. These tasks are quite time- consuming and require a large amount of technologist effort, a swell as a large number of rabbits. The treponemes needed for the various experiments proposed in the three projects can be provided much more economically, in terms of minimizing the number of rabbits as well as technologist time, if they are propagated centrally. Provision of antisera raised by the core to the project leaders will also assure uniformity of specificity, permitting comparison of results from one project to another. The core will be directed by Dr. Sheila Lukehart, who has 25 years of experience in the propagation of pathogenic treponemes.