CORE--DNA/PEPTIDE

寡核苷酸、多肽和蛋白质测序是现代分子医学和生物学的重要工具。获得这些工具对于调查和诊断与转化细胞状态和许多传染病有关的基因改变至关重要。DNA/多肽共享资源为癌症中心成员提供可靠、快速和负担得起的合成DNA和RNA(寡核苷酸)、多肽和蛋白质测序。寡核苷酸被用于DNA测序、聚合酶链式反应、定点突变和作为识别遗传信息的探针，修饰的寡核苷酸已被用于具有直接治疗可能性的反义实验。合成肽被用作探索蛋白质特定区域的功能或产生针对蛋白质特定区域的免疫制剂的直接手段。蛋白质测序用于确认蛋白质的识别或识别感兴趣蛋白质中的氨基酸序列区域。该设施以快速、高效的方式提供这些服务，将各个实验室的成本降至最低。它还为中心的研究人员提供了使用我们的仪器的低成本途径，如果他们开发需要的试剂或技术的话。该设施为癌症中心成员提供了一个信息源，帮助他们设计和实施特定类型的实验。工作人员可以对癌症中心的成员进行各种DNA/多肽技术的培训。

Oligonucleotides, peptides, and protein sequencing are essential tools of modern molecular medicine and biology. Access to these tools is essential for investigation and diagnosis of genetic alterations linked to transformed cell states and to many infectious diseases. The DNA/Peptide Shared Resource provides a dependable, rapid, and affordable supply of synthetic DNA and RNA (oligonucleotides), peptides, and protein sequencing for Cancer Center members. Oligonucleotides are used in DNA sequencing, as polymerase chain reaction primers, for site-directed mutagenesis, and as probes for the identification of genetic information Modified oligonucleotides have been used for anti-sense experiments with direct therapeutic possibilities. Synthetic peptides are used as a direct means of exploring the function of, or to generate immunological agents against, specific regions of proteins. Protein sequencing is used to confirm the identify of proteins or to identify regions of amino acid sequence in proteins of interest. This facility provides these services in a fast, efficient manner that minimizes the cost to individual laboratories. It also provides low cost access to our instrumentation for researchers at the Center, should they develop reagents or techniques requiring it. This facility serves as an information source for Cancer Center members to find assistance with the design and implementation of specific types of experiments. Staff members are available to train Cancer Center members in a wide variety of DNA/Peptide techniques.