CORE--BIOLOGICAL IMAGING ANALYSIS CORE

我们正在请求对生物成像核心的支持，该核心将继续 执行与此PPG相关的项目的基本功能 申请。基于PPG成员取得的显著进展 在过去的资助期内，很明显， 这项提案中的所有项目现在都处于这样一个点 形态相关性对于将生化和遗传 将信息转换为结构化的上下文。生物成像核心的巅峰 目的如下：第一，核心将提供技术支持 在准备、观看和解释薄片的所有方面都需要 切片电子显微镜。EM使用的一个主要方面将是 免疫细胞化学定位抗原技术的可行性 使用包埋前免疫金标记法和包埋后定位法 在冷冻薄片和亲水塑料切片上。第二， CORE将支持光镜免疫细胞化学的各个方面 这是分析细胞中表达的抗原的关键技术。 酷睿将通过使用BioRad MRC600提供实际帮助 激光扫描共聚焦显微镜和将继续协助 制定检查标记抗原和其他的程序 活细胞中的荧光探针。图像分析的方方面面 数字化和数据显示的重建、量化分析和 出版的准备工作可通过核心网站获得。最后，一个 核心意愿的重要方面是为互动提供重点 在这个PPG的调查人员中，并在很大程度上帮助 通过实验计划、执行和解释 合作。 Jamieson博士将担任该矿石的总负责人；每天 核心手术将由保罗·韦伯斯特博士进行，他是 精通材料的准备和分析的各个方面 光镜和EM免疫细胞化学。他将监督整个工作流程。 核心，由Linda Chicione女士协助，她提供一般技术 协助样品准备和暗室工作。菲利普·马尔·威尔先生 就共焦系统的使用提供工程支持和培训 和电子显微镜。所有与核心相关的专业人员都是 可提供有关实验方法、技术建议的咨询 以及PPG中大多数项目的合作者。最后，一个 工作人员的中心责任将是培训、监督和 新生和博士后研究员的质量控制 参与PPG项目。

We are requesting support for a Biological Imaging Core that will continue to perform an essential function for the projects associated with this PPG application. Based on the remarkable progress made by members of the PPG during the past grant period, it is clear that the research activities of all of the projects in this proposal are now poised at a point where morphological correlates are essential for placing biochemical and genetic information into a structural context. The Biological Imaging Core's prime purposes are as follow: First, the Core will provide technical support required for all aspects of preparation viewing and interpretation of thin section electron microscopy. A major aspect of EM use will be the availability of techniques for immunocytochemical localization of antigens using pre embedding immunogold labeling and post embedding localizations on frozen thin sections and sections of hydrophilic plastics. Second, the Core will support all aspects of light microscopic immunocytochemistry which is a critical technique for analysis of antigens expressed in cells. The Core will provide practical help with the use of the BioRad MRC 600 laser scanning confocal microscope and will continued to aid in the development of procedures for examination of tagged antigens and other fluorescent probes in live cells. All aspects of image analysis and reconstruction, quantitative analysis of digitized and data display and preparation for publication are available through the Core. Finally, an important aspect of the Core will to provide a focus for interaction amongst investigators in this PPG and to significantly aid in the planning, execution and interpretation of experiments through collaborations. Dr. Jamieson will serve as overall director of this ore; day to day operation of the Core will be carried out by Dr. Paul Webster who is conversant in all aspects of the preparation and analysis of material for light and EM immunocytochemistry. He will oversee the flow of work through the Core, assisted by Ms. Linda Chicione who provides general technical help with specimen preparation and darkroom work. Mr. Philippe Male will provide engineering support and training in the use of the confocal system and electron microscopes. All professionals associated with the Core are available for consultation on experimental approaches, technical advice and for collaborators on most of the projects in the PPG. Finally, a central responsibility of the staff will be training, supervision and quality control of new students and postdoctoral fellows who will participate in PPG projects.