DROSOPHILA PATTERN REPAIR: CELL FATE AND DEATH MAPPING

描述：明登博士有工具来监测 从有丝分裂区域出现的细胞。 这些域由 胚盘中同步分裂的细胞群， 预示着胚胎特定区域的发育。 他已经开发 基因表达和细胞死亡标记物，用于 活的发育中的胚胎 这些研究的核心是制作一个 GAL 4VP 16的“笼状”形式。 这涉及到一种光可逆的化学物质 蛋白质的修饰。 GAL 4VP 16暴露于 修饰赖氨酸的6-硝基藜芦基氯甲酸酯被证明可以阻断DNA 体外结合 将修饰的蛋白质注射到前胚盘中， 胚胎，并可以通过紫外线照射在单一的水平光活化 细胞目标。 他建议绘制出组成这12个细胞的所有细胞的命运， 有丝分裂域有助于头端区域。 有了这样的地图， 另一方面，将进行进一步的映射以确定细胞的模式 死亡在地图上。

DESCRIPTION: Dr. Minden has tools to monitor the developmental fates of cells emerging from mitotic domains. These domains are composed of synchronously dividing clusters of cells in the blastoderm, which appear to prefigure development of specific regions of the embryo. He has developed gene expression and cell death markers for use in time-lapse studies of live, developing embryos. Central to these studies has been production of a "caged" form of GAL4VP16. This involves a photoreversible chemical modification of the protein. Exposure of GAL4VP16 to 6-nitroveratrylchloroformate, which modifies lysine, was shown to block DNA binding in vitro. The modified protein is injected into pre-blastoderm embryos, and can be photoactivated by UV illumination at the level of single cell targets. He proposes to map the fates of all cells composing the 12 mitotic domains contributing to the procephalic region. With such maps in hand, further mapping will be carried out to determine patterns of cell death within the mapped domains.