EFFICACY STUDIES OF CHEMOPREVENTIVE AGENTS IN ANIMAL MOD

本项目的目的是验证两种与人类疾病具有组织学相似性的卵巢腺癌动物模型，以评估选定药物的化学预防效果。将7,12二甲苯[a]蒽浸透缝合线植入近交系8周龄Lewis大鼠和FVBxSwiss白化小鼠的左卵巢诱导卵巢癌。右卵巢将作为内部负性控制。将1 cm长的3-0缝线浸透2.1 fg的DMBA，用无菌针包埋于卵巢实质，一端打结固定。大鼠将在36周时被处死，用于评估卵巢癌症。这些小鼠将在30周时接受卵巢肿瘤评估。卵巢和肿瘤将固定在10%的缓冲福尔马林中，并由病理学家分析病变。每组将使用30只动物，这应该有足够的力量来观察治疗与控制的差异，卵巢癌减少50%。

The aim of this project is to validate two animal models of ovarian adenocarcinoma with histologic similarities to the human disease for the purpose of evaluating chemopreventive efficacy of selected agents. The ovarian cancers are being induced by implanting 7,12 dimethylbenz[a]anthracene impregnated suture into the left ovary of inbred 8-week old Lewis rats and FVBxSwiss albino mice. The right ovary will serve as a internal negative control. A 1 cm length of 3-0 suture is impregnated with 2.1 fg of DMBA and imbedded in the ovarian parenchyma with a sterile needle and knotted at one end to fix it in place. The rats will be sacrificed at 36 weeks for the evaluation of cancers of the ovary. The mice will be evaluated at 30 weeks for ovarian tumors. The ovaries and tumors will be fixed in 10% buffered formalin and analyzed by a pathologist for lesions. Thirty animals per group will be used which should allow sufficient power to see a difference in treatment versus control at 50% reduction in ovarian cancers.