PROSTAGLANDIN PRODUCTION BY PRIMATE PERIOVULATORY FOLLICLE

成功受精，当双亲基因组结合时完成 在激活的卵母细胞中，对高效 繁殖。日本血吸虫微管形态的研究 牛和猪的受精卵导致发现精子 在这些家畜受精过程中贡献了中心体 物种。此外，我们还证明了繁殖产生的精子 公牛在可预见的情况下组织微管的能力不同 精子掺入，以及精子中心体变异影响 受精的结果。该项目的目标是 在分子水平上表征相对亲本 受精过程中对牛受精卵的贡献=S中心体。 我们对这个项目有两个目标。目标1.是否招募 母体分子对精子中心体是必不可少的 受精完成了吗？目标2.分子基础是什么 团结男性和女性的动力是什么？这个 在这些实验中获得的信息将增加我们的 基础知识的分子机制导致 奶牛受精过程中的原核定位和基因组结合， 所有动物生殖过程中的关键一步。此外， 它为测试男性生殖功能的新方法带来了希望 在潜在的繁育种马中。这项研究还可能发现一种新的 检测影响生育能力的化合物的位置， 申请生育评估、生殖管理和 生殖毒理学，以及协助开发新的方法 以提高家养物种的繁殖效率。资金来源 美国农业部9702358出版物苏托夫斯基P，Simerly C，休伊特森L，Schatten G.核孔复合体和环状片层的组装促进 受精的哺乳动物卵母细胞的正常原核发育。J细胞 SCI 111：2841-2854,1998。

Successful fertilization, completed when parental genomes unite within an activated oocyte, is critically important for efficient reproduction. Investigations on the microtubule configurations in bovine and porcine zygotes have led to the discovery that the sperm contributes the centrosome during fertilization in these domestic species. In addition, we have demonstrated that sperm from breeding bulls vary predictably in the ability to organize microtubules after sperm incorporation, and that variations in sperm centrosomes affect the outcome of fertilization. The goals of this project are to characterize, at the molecular level, the relative parental contributions to the bovine zygote=s centrosome during fertilization. We have two aims for this project. Aim 1. Is the recruitment of maternal molecules to the sperm centrosome essential for the completion of fertilization? Aim 2. What is the molecular basis of the motility which unites the male and female pronu clei ? The information obtained during these experiments will add to our fundamental knowledge about the molecular mechanisms leading to pronuclear apposition and genomic union during fertilization in cows, a key step in the reproductive process of all animals. Furthermore, it holds promise for novel assays to test male reproductive function in potential breeding studs. This research may also uncover a new site at which to assay for compounds affecting fertility, with applications for fertility assessment, reproductive management, and reproductive toxicology, as well as aid in developing new approaches for improving reproductive efficiency in domestic species. FUNDING USDA 9702358 PUBLICATIONS Sutovsky P, Simerly C, Hewitson L, Schatten G. Assembly of nuclear pore complexes and annulate lamellae promotes normal pronuclear development in fertilized mammalian oocytes. J Cell Sci 111:2841-2854, 1998.