PILOT & FEASIBILITY STUDIES

甲状腺功能下降常发生在危重病人身上 具有预后意义。 然而，目前尚不清楚， 甲状腺功能减退是器官功能障碍的原因，或者只是一种 急性脓毒症的表观现象。 本研究的目的是 测定外源性甲状腺激素改善肝脏功能的能力 脓毒症大鼠模型中的生物能量学。 生物能量功能将是 通过测量肝脏中的高能磷酸盐水平进行评估 使用31 P NMR光谱。 研究中的实验动物 将在脓毒症后立即接受甲状腺治疗 诱导 高能磷酸盐水平的比较 治疗组和非治疗组将证明 甲状腺功能对肝脏生物能量学的影响初步数据显示 一个受伤但未受攻击的肝脏表现出明显的稳定性， 通过ATP和无机磷酸盐（Pi）评估的生物能量功能 浓度的 随后，当肝脏受到 代谢挑战（胰高血糖素），Pi/ATP增加 比例 这一比率的增加表明， 从能量学的角度来看，肝脏功能失调。 我们 初步数据（无胰高血糖素激发）未 证明了败血症动物的肝脏在生物能量上 不稳定所以我们用胰高血糖素激发试验了肝脏 这些数据的初步结果表明， 败血症动物的生物能量衰竭，而非败血症动物则 没有 因此，我们现在建议研究 三碘甲状腺原氨酸（T3）替代对改变这种肝脏 功能障碍 :

Decreased thyroid function often occurs in critically ill patients and is of prognostic significance. However, it is unknown if hypothyroidism is a cause of organ dysfunction or just an epi-phenomena of acute sepsis. The objective of this study is to determine the ability of exogenous thyroid hormone to improve hepatic bioenergetics in a septic rat model. Bioenergetic function will be assessed by measuring the high energy phosphate levels in the liver using 31P NMR spectroscopy. The experimental animals in the study will be given thyroid treatment immediately following septic induction. The comparison of the high energy phosphate levels between the treatment and non-treatment groups will demonstrate the influence of thyroid function on hepatic bioenergetics Preliminary data show that an injured but unchallenged liver exhibits apparently stable bioenergetic function as assessed by ATP and inorganic phosphate (Pi) concentrations. Subsequently, when the liver is subjected to a metabolic challenge (glucagon), there is an increase in the Pi/ATP ratio. This ratio increase demonstrates the severity to which the liver is dysfunctional from an energetics perspective. Our preliminary data (without the glucagon challenge) have not demonstrated the livers of the septic animals to be bioenergetically unstabIe~therefore, we tested the liver with a glucagon challenge. The preliminary results from these data demonstrate hepatic bioenergetic failure in septic animals, whereas non-septic animals do not. Accordingly, we now propose to study the effects of triiodothyronine (T3) replacement on modifying this hepatic dysfunction. :