SINGLE PHOTON COUNTING FLUORESCENCE & IMAGING APPARATUS

时间相关单光子计数（TCSPC）荧光 该装置作为现有技术的光谱仪器来执行。 在 雅阁的目标是开发新的技术，从现有的 仪器，我们已经开发了一个时间分辨共焦光学 光谱仪，彻底改变了分子动力学的研究， 通过将空间耦合 分辨率与国家的最先进的超快光谱方法。 我们 仪器设计用于采集单个位置或扫描图像， 使用三种对比机制（力、强度和激发） 状态寿命）。 在过去 今年，我们已经更新了我们的共聚焦显微镜，以双通道操作 其同时允许在两个单独的光子探测器中探测光子， 光谱区 这使我们能够监控 供体/受体对之间的共振荧光能量转移 许多不同的系统;在最近的研究中，GCN-4 肽和细胞内蛋白质。 其他事态发展 包括将常规TCSPC系统与 共焦光谱仪 这种分离检测和 电子设备，这将有助于最大限度地利用这两种仪器，是 几乎完成。 只有更新和编程的一个单独的 计算机数据采集中心（具有更快的处理器）， TCSPC仍有待完成。

The time-correlated single photon counting (TCSPC) fluorescence apparatus performs as a state-of-the art spectroscopic instrument. In accord with the goal of developing new technologies out of existing instrumentation we have developed a time resolved confocal optical spectrometer which revolutionizes the study of molecular dynamic and photonic processes by incorporating the coupling the spatial resolution with state-of-the-art ultrafast spectroscopic methods. Our instrument is designed to acquire single location or scanned images of samples using three contrast mechanisms (force, intensity and excited state lifetime) in quickly converting configurations. Within the past year we have updated our confocal microscope to two channel operation which simultaneously allows detection of photons in two separate spectral regions. This allows us the capability of monitoring resonance fluorescence energy transfer between donor/acceptor pairs in many different systems; among those recently studies are tagged GCN-4 peptides and intracellular proteins in vivo. Other developments include the separation of the conventional TCSPC system from the confocal optical spectrometer. This separation in detection and electronics, which will facilitate maximum use of both instruments, is nearly complete. Only the updating and programming of a separate computer data acquisition center (with a faster processor) for the TCSPC remains to be completed.