GENETIC REGULATION OF ACETYLCHOLINE SYNTHESIS

描述：拟议研究的长期目标是确定 土壤胆碱能神经元功能的遗传和分子基础 线虫。本研究的重点是 胆碱能基因，包含2个基因：CHA-1是结构基因 对于胆碱乙酰转移酶(ChAT)，合成 神经递质乙酰胆碱，UNC-17是编码 突触囊泡乙酰胆碱转运体。本研究主要有6个方面 目标： 1)研究大脑皮层胆碱能神经系统的发育 通过监测胚胎和胚胎后发育的外观 胆碱能标志物。2、3)确定参与 胆碱能表型的控制和表达。4、5)研究 特定类别的胆碱能神经元对整体的贡献 神经系统的功能和有机体的行为。6)至 研究神经元递质在神经发育过程中的作用 神经元。 这项拟议的研究将提供一项分子和基因分析， 神经元成为胆碱能神经元，应该通过以下方式来阐明这一过程 哪些神经元分化并实现共同的神经递质 身份。

DESCRIPTION: The long-term goal of the proposed research is to determine the genetic and molecular bases of cholinergic neuronal function in the soil nematode Caenorhabditis elegans. The focus of the present research is on the cholinergic locus, which contains 2 genes: cha-1 is the structural gene for choline acetyltransferase (ChAT), the enzyme which synthesizes the neurotransmitter acetylcholine, and unc-17 is the gene which encodes the synaptic vesicle acetylcholine transporter. The research has 6 primary aims: 1) To study the development of the cholinergic nervous system during embryonic and postembryonic development by monitoring the appearance of cholinergic markers. 2, 3) To identify trans-acting factors involved in the control and expression of the cholinergic phenotype. 4, 5) To study the contribution of specific classes of cholinergic neurons to the overall function of the nervous system and behavior of the organism. 6) To investigate the role of a neuron's transmitter on the development of the neuron. The proposed research will provide a molecular and genetic analysis of how neurons become cholinergic neurons and should elucidate the processes by which neurons become differentiated and achieve a common neurotransmitter identity.