SURFACE MODIFICATION FOR SELECTIVE PROTEIN ADSORPTION

下一代医用植入物的发展 包括将适当的生物识别分子附着在适当的 在植入物表面的取向和浓度，以防止 导致异物反应的细胞激活和 植入物封闭术。卟啉分子是理想的 开发具有可控、最佳间距的均匀单分子膜 生物识别小组的成员。金表面自组装的卟啉单分子膜 表面用三种定制合成的烷基硫醇取代 本课题组已开发出四苯基卟啉分子。 几种技术包括X射线光电子能谱(XPS)， 紫外/可见光吸收光谱(UV/Vis)，扫描 隧道显微镜(STM)和掠角红外光谱 (GAIR)用于表征单分子膜。XPS和GAIR 到目前为止的研究表明，卟啉是化学吸附在 表面通过硫金键，并且卟啉分子 在黄金表面上以并排的方向排列。盖尔 使用偏振光源的结果表明，卟啉 戒指的方向与金色表面平行。

The development of the next generation of medical implants involves attaching appropriate biorecognition molecules in the proper orientation and concentration on the surface of an implant, to prevent the cellular activation that leads to the foreign body response and implant encapsulation. Porphyrin molecules are ideal for the development of a uniform monolayer, with controlled, optimal spacing of biorecognition groups. Self-assembled porphyrin monolayers on gold surfaces with three custom synthesized alkylthiol substituted tetraphenylporphyrin molecules have been developed by our group. Several techniques including x-ray photoelectron spectroscopy (XPS), ultraviolet/visible absorption spectroscopy (UV/Vis), scanning tunneling microscopy (STM), and grazing-angle infrared spectroscopy (GAIR) are being used to characterize the monolayers. XPS and GAIR studies to date reveal that the porphyrins are chemisorbed to the surface through a sulfur-gold bond, and that the po rphyrin m olecules are aligned on the gold surface in a side-by-side orientation. GAIR results using a polarized light source indicate that the porphyrin rings are oriented parallel to the gold surface.