NERVE-MUSCLE INTERACTIONS DURNIG JAW MUSCLE DEVELOPMENT

描述（改编自研究者摘要）：神经的作用 哺乳动物发育和分化中的肌肉相互作用 咀嚼肌肉和神经元组织的模式， 很少受到关注。 然而，许多研究都集中在 神经-肌肉相互作用在肢体肌肉发育中的作用。 先前 对肢体的研究已经确定，运动神经元和肌肉， 受神经支配动物的正常发育相互依赖， 维护和它们的生理特性。 虽然有价值的线索， 神经-肌肉相互作用在发育和维持中的作用 咀嚼肌可以从四肢肌肉的研究中收集， 肢体研究的结果可能不完全适用。 的肌肉 咀嚼肌与四肢肌在胚胎起源上不同， 感觉和运动神经元支配他们和独特的 在成人中表达的纤维类型。 此外，理解 参与口面形态发生的发育过程是必需的， 了解成年人的形式和功能，并可能提供有价值的 深入了解在修改成人的过程中可能遇到的限制 form. 因此，本提案的重点是调查 咀嚼肌和三叉神经的时空发育 复杂，以提高我们对神经肌肉相互作用的理解， 发展 本研究提出两个假设以供验证 并重点研究三叉神经中枢神经系统的组织、咀嚼肌 肌肉发育和神经-肌肉相互作用。 第一个假设是 三叉神经系统的中枢成分参与神经支配 包括motV和mesV在内的咀嚼肌， 时空序列和响应来自其目标的线索。 的 第二个假设是咀嚼肌纤维的成人表型 和肌肉纤维分布在每个颌骨肌肉是依赖于 三叉神经肌肉相互作用 这两个假设将被检验 在三个具体的目标，包括在体内和体外研究。

DESCRIPTION (Adapted from investigator's Abstract): The role of nerve muscle interactions in the development and differentiation of mammalian muscles of mastication and the patterning of neuronal organization has received little attention. However, much research has concentrated on the role of nerve-muscle interactions in limb muscle development. Previous studies in the limb have determined that motoneurons and the muscles they innervate are dependent on one another for their normal development, their maintenance and their physiological properties. Although valuable clues as to the role of nerve-muscle interactions in the development and maintenance of masticatory muscles can be gleaned from studies of limb muscle, the result of limb studies may not be totally applicable. The muscles of mastication differ from limb muscle in their embryonic origin, the origin of the sensory and motor neurons innervating them and in the uniqueness of the fiber types expressed in the adult. Moreover, an understanding of developmental processes involved in orofacial morphogenesis is required for an understanding of the adult form and function, and may provide valuable insight into what limitations might be encountered in modifying the adult form. Therefore, the focus of this proposal is to investigate the temporospatial development of the masticatory muscles and the trigeminal complex to enhance our understanding of nerve-muscle interactions during development. Two hypotheses are proposed to be tested during this project and focus on trigeminal central nervous system organization, masticatory muscle development and nerve-muscle interactions. The first hypothesis is that central components of the trigeminal system involved in the innervation of the muscles of mastication including motV and mesV, develop in distinct temporospatial sequences and in response to cues from their targets. The second hypothesis is that the adult phenotype of masticatory muscle fibers and the muscle fiber distribution within each jaw muscle are dependent on trigeminal nerve-muscle interactions. These two hypotheses will be tested in three specific aims that include in vivo and in vitro investigations.