ACQUISITION OF MIRCOARRAY SPOTTER, READER, SOFTWARE

描述：（调查员？s摘要）：本申请要求资金， 购买一个观察器，阅读器和相关的软件，以建立一个DNA 加州大学戴维斯分校的微阵列中心。该中心将为18个NIH资助的 调查人员的技术和设备所需的产生定制 DNA芯片此外，三个中心（超级基金，NIEHS中心， 农业化学品和加州灵长类动物研究中心）和学生 在一些NIH支持的培训项目中（ 环境毒理学、环境病理学、肺医学）将 从使用这些设备中获益。当EST集合被建立并且 所需的专业知识和设备的可及性变得更大，我们 预计将有更多的用户比目前列出的 应用程序. 最近引进的高通量技术， 表达式分析为理解 细胞对各种操作的反应的多样性和复杂性。在 此外，这些方法还允许快速和更详细地查看 基因表达模式对疾病的反应发生改变， 大大加快了对潜在药物靶点的鉴定， 这些疾病的治疗。虽然一些研究问题可以 答案是使用DNA阵列，获得设备和成本， 商业阵列阻碍了这些方法的充分应用， investigators.主要用户在此应用程序中概述的大部分工作 聚焦于靶组织/细胞的变化，包括肺细胞， 生殖系统、心血管系统、神经系统和皮肤系统 一群不同的特工在所有这些研究中，目的是确定 变化，这发生在基因调控响应毒物，并作为一个 导致理解导致毒性的关键事件。第二 一组研究人员将应用这些方法来研究 对包括精神分裂症在内的疾病以及与 对病毒感染的易感性。在所有情况下， 用于产生足够数量的芯片的灵活技术， 对转录调控中发生的变化进行详细、彻底的分析 都将扩大这群人可能提出的问题， 调查人员，并将提高他们可以回答的速度。

DESCRIPTION: (Investigator?s Abstract): This application requests funds to purchase a spotter, reader and associated software for establishing a DNA microarray center at UC Davis. The center will provide access for 18 NIH funded investigators to the technology and equipment necessary for generating custom DNA chips. In addition, three centers (Superfund, the NIEHS Center for Agricultural Chemicals and the California Primate Research Center) and students in a number of NIH supported training programs (training grants in Environmental Toxicology, Environmental Pathology, Pulmonary Medicine) will benefit from access to this equipment. As EST sets are established and accessibility of the expertise and equipment needed become greater, we anticipate that there will be more users than listed in the current application. The recent introduction of high throughput techniques for genome wide expression analysis has opened a number of opportunities to understand the variety and complexity of cellular responses to various manipulations. In addition, these approaches have allowed a rapid and more detailed view of alterations in gene expression patterns in response to diseases, which in turn has greatly accelerated the identification of potential drug targets for the treatment of these diseases. While a number of research questions can be answered by the use of DNA arrays, access to the equipment and the cost of commercial arrays has hampered full application of these approaches by many investigators. Much of the work outlined by the major users in this application focuses on changes in target tissues/cells including cells of the pulmonary, reproductive, cardiovascular, neurologic and dermatologic systems in response to a diverse set of agents. In all of these studies the intent is to identify changes, which occur in gene regulation in response to the toxicant, and as a lead toward understanding the critical events leading to toxicity. A second group of investigators will apply these approaches to study of alterations in response to diseases including schizophrenia as well as those associated with susceptibility to viral infections. In all cases the ready availability of flexible technology for generation of sufficient numbers of chips to allow detailed, thorough analysis of changes occurring in transcriptional regulation will both broaden the questions that can be posed by this group of investigators and will increase the speed with which they can be answered.