CONFOCAL MICROSCOPE FOR LIVE CELL & MULTIWAVELENGTH WORK

了解基因功能的许多进展都来自于发现它们的同源物在模式遗传生物中的作用。拟议用户研究的遗传和基因组学模式生物包括酵母、动物果蝇、秀丽隐杆线虫、鸡和小鼠以及植物Arabadopsis。北卡罗来纳大学教堂山分校已经认识到现代生物学的这些发展，并创建了一个整合动物和植物分子生物学、结构生物学、细胞生物学、发育生物学、显微镜和成像、生物分析化学和计算等专业知识的倡议。文理学院的重点是使用非哺乳动物模式生物，而医学院的一个补充计划则侧重于使用小鼠建立疾病模型。这些举措将共享技术，创造显著的规模经济，并提供重要的智力交流和尖端教育项目的发展。生物系是文理学院的重点；我们在现代生物学中使用的大多数模式生物方面都有显著的优势。我们综合方法的一个基石是使用生物成像来揭示亚细胞蛋白质，并及时跟踪这些蛋白质在活生物体中的变化。这将需要对细胞、组织和完整生物体中的分子进行4d成像（3D加时间）。近年来，荧光显微镜和数字成像技术取得了巨大的进步，它们与绿色荧光蛋白（GFP）的结合将对细胞和组织中蛋白质表达和功能的分析产生巨大的影响。多波长荧光显微镜的新技术将允许在细胞、组织或整个模式生物中跟踪多种蛋白质的表达、动态和相互作用。多细胞生物将需要特殊技术来获得组织深处的图像，这是该部目前使用的共聚焦和宽视场荧光显微镜无法获得的。为了获得最大的效果，显微镜需要靠近研究实验室，在附近的成像设备中。我们的建议旨在通过创建一个多用户设施来满足这些新的需求，该设施将为NIH资助的一大群研究人员提供最先进的共聚焦显微镜。

Many advances in understanding gene function have come from discovery of the roles of their homologs in model genetic organisms. Genetic and genomics model organisms, studied by the proposed users include yeast, the animals Drosophila, C. elegans, chickens and mice and the plant Arabadopsis. The University of North Carolina at Chapel Hill has recognized these developments in modern biology, and has created an initiative integrating expertise in animal and plant molecular biology, structural biology, cell biology, developmental biology, microscopy and imaging, bioanalytical chemistry and computing. Emphasis in the College of Arts and Science is o the use of non-mammalian model organisms, while a complementary initiative in the School of Medicine focuses on modeling disease using the mouse. These initiatives will share technologies and create significant economies of scale as well as providing important intellectual cross-fertilization and the development of cutting-edge educational programs. The Departmental of Biology is at the effort in the College of Arts and Science; we have significant strengths in most model organisms in use in modern biology. One cornerstone of our integrated approach is the use of biological imaging to reveal the subcellular proteins, and to follow changes in these proteins in time in living organisms. This will require 4-D imaging (3D plus time) of molecules in cells, tissues, and intact organisms. There have been tremendous advances in flourescent light microscopy and digital imaging recently which, in combination with the green flourescent protein (GFP) will have tremendous impact on assaying protein expression and function in cells and tissues. New technologies for multi-wavelength fluorescence microscopy will allow the expression, dynamics and interactions of multiple proteins to be followed within cells, tissues or whole model organisms. Multicellular organisms will require special techniques for obtaining images deep within tissues not available from the current confocal and wide field fluorescence microscopes in the Department. For maximum effectiveness, the microscopes need to be located close to research labs, in a nearby imaging facility. Our proposal seeks to meet these new needs, by the creation of a multi-user facility that will provide a state-of-the art confocal microscope to a large group of NIH funded investigators.