MULTIUSER AFFYMETRIX GENE CHIP SYSTEM

描述：(调查员？S摘要)：这是一项建立一个 全面的DNA微阵列分析和生物信息学系统 肯塔基大学。DNA微阵列方法显然将带来革命性的变化 未来对基因表达的研究。这里已经有许多NIH支持的项目 关注基因表达和调控方法，在许多情况下 转基因动物。因此，整合大规模基因的能力 数组分析将极大地加快进度，改进解释，并 推动在这些领域中发现新的表达-功能相关性 项目。目前，在这里进行表情分析的是慢， 劳动密集型和/或有限的方法(例如，聚合酶链式反应，小芯片，北方人， 等)。基因阵列能力也将在肯塔基州带来特别的好处 因为很多主要的用户？项目强调量化比较 以及检测基因表达的细微差异(例如，随着年龄的增长， 暴露于生长因子、激素或毒物、脑损伤、昼夜节律 节律、致癌、信号级联等)。集成系统 此处要求(Affymetrix)，是唯一全面的系统(芯片、 杂交炉、流体站、激光分析仪、生物信息学包) 目前可供使用，我们的几名教员在使用它方面经验丰富。 这个系统似乎也是最灵敏、准确和标准化的， 将成为量化导向的关键优势的特征 肯塔基大学的项目。此外，有几个项目位于 肯塔基州正在开发和完善用于基因表达研究的新方法 功能特征单一或同源细胞，以获得更多 准确研究表情和功能之间的关系。的一个组件 这项工作涉及到足够数量的代表性放大 允许在单个细胞中进行表达谱分析。这些方面的发展 基因阵列图谱将极大地促进方法，这些基因阵列可以 提供关于如何代表性和可重现性的详细信息 扩增程序均为。因此，拟议的系统不仅将 具体加强美国国立卫生研究院资助的多个大学项目的进展 ，但将促进新方法的开发，这种方法很可能 许多其他机构的调查人员也从中受益。

DESCRIPTION: (Investigator?s Abstract): This is a proposal to establish a comprehensive DNA Microarray Analysis and Bioinformatics System at the University of Kentucky. DNA microarray methods clearly will revolutionize future studies of gene expression. Numerous NIH-supported projects here already focus on gene expression and regulation approaches, in many cases with transgenic animals. Therefore, the ability to incorporate large-scale gene array analyses would vastly accelerate progress, refine interpretation, and drive the discovery of novel expression-function correlations in these projects. At present, expression analyses are performed here with slow, labor-intensive and/or limited methods (e.g., PCR, small chips, Northerns, etc.). Gene array capability would also be of particular benefit at Kentucky because many of the major users? projects emphasize quantitative comparisons and detection of subtle differences in gene expression (e.g., with aging, exposure to growth factors, hormones or toxicants, brain injury, circadian rhythms, carcinogenesis, signaling cascades, etc). The integrated system requested here (Affymetrix), is the only fully comprehensive system (chips, hybridization oven, fluidics station, laser analyzer, bioinformatics package) presently available, and several of our faculty are experienced in its use. This system also appears to be the most sensitive, accurate and standardized, characteristics that would be a key benefit to the quantitatively oriented projects at the University of Kentucky. In addition, several of the projects at Kentucky are developing and refining novel methods for expression studies in functionally characterized single orhomogenous cells in order to more accurately study correlations between expression and function. A component of this work involves the representative amplification of sufficient quantities of mRNA to allow expression profiling in single cells. Development of these approaches would be greatly facilitated by gene array profiles that could provide detailed information on how representative and reproducible the various amplification procedure are. Thus, the proposed system would not only specifically enhance progress in multiple NIH-funded projects at the University of Kentucky, but would facilitate the development of new methods that may well benefit investigators at many other institutions as well.