Core--Molecular biology and cell culture

分子生物学和细胞培养核心将提供一个集中设施，用于进行所有定点诱变实验、构建新的表达质粒以及创建表达四个项目的PI所需的预期蛋白质的细菌和幼仓鼠肾细胞系。将与适当的P.I.协商构建用于表达新丝氨酸蛋白酶抑制剂的质粒。核心将与蛋白表达和纯化核心占据共同空间。行政主任将负责核心项目的通宵工作，并确保为所有项目提供公平的服务。

The Molecular Biology and Cell Culture Core will provide a centralized facility for carrying out all site-directed mutagenesis experiments, construction of new expression plasmids and creation of bacterial and baby hamster kidney cell lines expressing the desired proteins required by the P.I.s of the four projects. Construction of plasmids for e3xpression of new serpins will be done in consultation with the appropriate P.I. The core will occupy common space with the Protein Expression and Purification core. The Administrative Director will be responsible for overnight of the core and for ensuring equitable service to all of the projects.