HEMATOPOIETIC STEM CELL GROWTH AND ENGRAFTMENT

马萨诸塞州大学医学院的合作研究人员 中心和耶鲁大学医学院共同开发了一种 造血干细胞的生长和分化。 的 研究主题集中在造血干细胞的遗传特征 细胞表型及细胞周期时相与干细胞的关系 表型特性，重点是在体内植入， 骨髓清除小鼠模型。 这些主题结合在一起，努力改善 新载体中的基因体外转移至造血干细胞 反过来，在旨在改善基因携带者植入的研究中， 干细胞 该计划项目被组织成一个团队的方法， 综合追求5个项目，2个研究支持核心和一个 行政核心。 个别项目涉及查明 原始干细胞中的关键基因和基因以及转录 决定更新、细胞周期进程和 造血干细胞的移植潜能， 它涉及细胞周期状态和细胞因子刺激;并且， 开发新的抗HIV载体，用于整合到 增殖性和非增殖性干细胞， 地位，并加强移植。 两个核心实验室支持分子 遗传学和干细胞纯化。 干细胞纯化核心是一个 尤其是耶鲁大学和UMMC之间的重要联系。 本方案项目 应用程序汇集了来自两个机构的研究人员， 基础和临床种类繁多的部门和部门 研究专长，以解决干细胞生物学的基础问题， 为临床基因治疗的发展奠定了基础。

Collaborating investigators at the University of Massachusetts medical Center and Yale University School of Medicine have jointly developed a Program Project on hematopoietic stem cell growth and differentiation. The research themes focus on genetic characterization of the hematopoietic stem cell phenotype and the relationship of cell cycle phase to stem cell phenotypic properties with an emphasis on in vivo engraftment in a myeloablated mouse model. These themes coalesce in efforts to improve the transfer of genes in vitro in novel vectors to hematopoietic stem cells and, in turn, in studies designed to improve engraftment of gene-carrying stem cells. This Program Project is organized into a team approach for the integrated pursuit of 5 projects, 2 research support cores and an administrative core. The individual projects address the identification of critical genes in primitive stem cells and genes as well as transcription factors defining decisions between renewal, cell cycle progression and differentiation; the engraftment potential of hematopoietic stem cells as it relates to cell cycle status and cytokine stimulation; and, the development of novel anti-HIV vectors for integration into both proliferating and non-proliferating stem cells to manipulate proliferative status and to enhance engraftment. Two core laboratories support molecular genetics and stem cell purification. The stem cell purification core is a particularly critical link between Yale and UMMC. This Program Project application brings together investigators from two institutions, from many departments and divisions with a wide variety of both basic and clinical research expertise to address basis problems in stem cell biology an to create a viable base for the development of clinical gene therapy.