Toxokinetics of Swainsonine--Nutrient Metabolism Effects

目标和目的：该项目的长期目标包括：1)培养学生的毒理学和营养代谢的原理，2)确定动力学和模式的马豆素的吸收，3)确定马豆素对营养代谢的影响。为了实现这些目标，本项目将解决四个具体目标。具体目标#1是确定马豆素的吸收动力学具体目标#2是确定马豆素对营养物质消化和通过消化道流动的影响。具体目的#3是确定马豆素的肌肉组织通量。具体目标#4是确定马豆素对选定营养素的肌肉组织通量的影响。苦马豆素是一种天然植物。临床上，马豆素已显示出作为抗肿瘤药物的前景。因此，马豆素作为一种抗肿瘤药物已显示出前景。因此，人们正在积极探索马豆素作为癌症的治疗方法。尽管人们对苦马豆素的化疗特性进行了广泛的研究，但对苦马豆素的药代动力学特性进行了广泛的研究的数据很少或根本没有，如果我们要制定口服剂量方案和营养支持策略，那么苦马豆素的药代动力学或苦马豆素的作用或对营养代谢的影响的数据很少或根本没有。动物将被手术插管/导管，以方便收集消化和血液样本进行马豆素分析和营养代谢物分析。此外，将收集相同的肠上皮细胞进行微泡转运研究。这些研究将提供有关马豆素吸收及其对营养代谢影响的一些初步数据。

Goals and Aims: The long term goals of this project include: 1) training students in the principles of toxicology and nutrient metabolism, 2) defining the kinetics and mode of swainsonine absorption, and 3) defining the effects of swainsonine on nutrient metabolism. In order to accomplish these goals, four specific aims will be addressed in this project. Specific Aim #1 is to define the absorption kinetics of swainsonine Specific Aim #2 is to define the effects of swainsonine on nutrient digestion and flow through the digestive tract. Specific Aim #3 is to define the spanchnic tissue flux of swainsonine. Specific Aim #4 is to define the effects of swainsonine on spanchnic tissue flux of selected nutrients. Swainsonine is a naturally occurring plant. Clinically, swainsonine has shown promise as an anti-tumor agent. As such, swainsonine has shown promise as an anti-tumor agent. As such, swainsonine is actively being explored as a treatment for cancer. Although the chemotherapeutic properties of swainsonine have been extensively studied, little or no data exists concerning the pharmacokinetics properties of swainsonine have been extensively studied, little or no data exists concerning the pharmacokinetics of swainsonine or the effects of swainsonine or the effects on nutrient metabolism if we are to develop oral dosing protocols and nutritional support strategies for the use of swainsonine as a cancer treatment. Animals will be surgically cannulated/catheritized to facilitate the collection of digest and blood samples for swainsonine analysis and nutrient metabolite analysis. In addition, same intestinal epithelia will be collected for microvesicle transport studies. These studies will provide some of the first data concerning the absorption of swainsonine and its effects on nutrient metabolism.