CORE--VIRUS AND CELL BIOLOGY

病毒和细胞生物学核心[VCBC]的目的是支持 项目1-4在拟议的计划项目补助金[PPG]。VCBC将 参与本PPG中提议的以下研究：（1）。的 从尿液中鉴定作用物的作用机制 表现出抗KS活性的孕妇;（2）.分离和 孕妇尿液中表现出以下特征的因素的表征 （3）Kaposi肉瘤（KS）与肿瘤生长的关系 人类疱疹病毒HHV 8是一种潜在的致病因子;（4）.和 KS细胞系的分子决定因素。VCBC一直积极 参与开发艾滋病的体外分析和细胞模型， 具有设计用于研究各种 KS方面。VCBC将提供集中的服务和生物 PPG中项目1-4的样品，以确保一致的质量， 试剂和测定的均匀性。VCBC的目标， 六个具体目标，是：接收和监测库存水平 PPG中的所有生物材料，并保持所有生物 材料在最佳储存条件下;提供试剂[病毒， 细胞、单克隆抗体和合成肽]对个体 研究人员和进行生物测定，以进一步的具体目标， 在个别项目中建议进行的调查; 动物核心设施[核心B]，用于向项目1-4报告。 维持材料，提供病毒储备和细胞， 和/或通过VCBC协调生物测定将确保 本PPG所有项目的生物测定均采用 筛选，标准化的试剂，从而促进之间的比较 所有项目生成的数据集。

The purpose of the Virus and Cell Biology Core [VCBC] is to support Projects 1-4 in the proposed Program Project Grant [PPG]. The VCBC will participate in the following investigations proposed in this PPG: (1). The identification of mechanisms of action of actors from the urine of pregnant women that exhibit anti-KS activity; (2). The isolation and characterization of factors from the urine of pregnant women that exhibit anti-KS activity; (3) The relationship between Kaposi's sarcoma (KS) and the human herpesvirus HHV8 as a potential etiologic factor; (4). And the molecular determinants of KS cell lines. The VCBC has been actively involved in developing in vitro assays and cellular models for AIDS and has experience working with in vitro assays designed to study various aspects of KS. The VCBC is to provide centralized services and biological samples for Projects 1-4 in the PPG and to assure consistent quality and uniformity of reagents and assays. The VCBC goals, which are incorporated into six Specific Aims, are: to receive and monitor the inventory levels of all biological material in the PPG and to maintain all biological materials at optimal storage conditions; to provide reagents [viruses, cells, monoclonal antibodies, and synthetic peptides] to individual investigators and to perform bioassays to further the Specific Aims of the investigations proposed in the individual projects; to collate data with the Animal Core Facility [Core B] for reporting back to Projects 1-4. Maintaining materials, providing virus stocks and cells, and performing and/or coordinating bioassays through the VCBC will ensure that the biological assays for all the projects of this PPG are conducted with screened, standardized reagents, thus facilitating comparisons among the data sets generated by all the projects.