Core--Xiphophorus Genetic Stock Center

产品描述：（申请人的描述）西南德克萨斯州立大学的剑尾鱼遗传资源中心将作为该计划项目的核心设施，因为（1）该计划项目中所需的杂交和回交杂交鱼生产所需的所有资源都作为纯种系保存在资源中心，（2）库存中心是两个研究机构中唯一有能力容纳和喂养估计2,000只动物的设施，这些动物将在估计的2,000年期间每年繁殖，000只动物，在项目过程中，每年将繁殖，和（3）建议的实验治疗与紫外线和MNU发生在早期的生活回交杂交，因为实验动物必须保持六个月或更长时间之前，牺牲，股票中心是最合理的设施总部的核心。核心项目的具体目标是确保（1）生产杂交种所需的亲本种群保持足够的数量，（2）所有杂交产生足够的F1后代用于回交交配，（3）回交后代的数量足以满足试验和对照的要求，数量足以满足每个项目的试验和对照要求（4）回交后代接受精确的实验处理，即，每个项目研究者指定的UV照射或MNU剂量，（5）整个动物群体的健康从未受到损害，以及（6）这些实验中使用的谱系的遗传背景和储备纯度得到充分表征。基线数据的遗传背景的父母和F1杂交股票用于制造回交杂交是必要的，特别是在最近的研究结果表明，股票同质性的假设，也必须在剑尾鱼股票的等基因克隆斑马鱼股票的polyallelism。每个种群的生化遗传图谱将确定每个组成项目所需的额外杂交交配的候选者，以继续其连锁图谱饱和和/或相关的肿瘤标志物图谱绘制工作。在每次交配结束后，核心机构人员将对该计划中使用的所有育雏的父母进行50个以上蛋白质编码位点的筛查，并将对该计划项目中使用的动物的兄弟姐妹进行筛查。为该计划项目建立一个核心设施的意义在于，它将集中管理牲畜、亲本、杂交和回交动物产品、实验处理、动物核心和牲畜纯度，从而简化该计划项目的后勤工作，大幅降低研究成本。

Description: (Applicant's Description) The Xiphophorus Genetic Stock Center at Southwest Texas State University will serve as the core facility for the Program Project because (1) all stocks necessary for the production of hybrid and backcross hybrid fishes required in the Program Project are maintained as pedigreed lines at the Stock Center, (2) the Stock Center is the only facility within the two research institutions that has the capacity to house and feed the estimated 2,000 animals that will be bred per year during the course of the estimated 2,000 animals that will be bred per year during the course of the project, and (3) proposed experimental treatments with UV and MNU occur early in the life of the backcross hybrids, and since experimental animals must be maintained for six months or more prior to sacrifice, the Stock Center is the most logical facility for headquartering the core. The specific aims of the Core Project are to ensure that (1) parental stocks required to produce hybrids are maintained in sufficient numbers, (2) all hybridizations produce sufficient F1 progeny for backcross matings, (3) backcross offspring are produced in sufficient numbers for the experimental and control of requirements produced in sufficient numbers for the experimental and control requirements of each project (4) backcross offspring receive the precise experimental treatment, i.e., UV irradiation or MNU dosage specified by each project investigator, (5) the health of the entire animal colony is never compromised, and (6) the genetic backgrounds and stock purity of pedigrees used in these experiments are well characterized. Baseline data regarding the genetic backgrounds of parental and F1 hybrid stocks used to manufacture backcross hybrids are necessary, particularly in light of recent findings of polyallelism in isogenic clonal zebrafish stock which suggest that assumptions of stock homogeneity must also be validated in Xiphophorus stocks. Biochemical genetic profiles for each of the stocks will identify candidates for additional hybridization mating required by each component project to continue their linkage map saturation and/or associated tumor marker mapping efforts. Core facility personnel will screen parents of all broods utilized in the Program for more than 50 protein-coding loci after each mating has been terminated and will screen siblings of the animals used in the Program Project. The significance of establishing a core facility for the Program Project is that it will centralize stock management, parental, hybrid, and backcross animal product, experimental treatment, animal core, and stock purity, thereby streamlining the logistics of the Program Project and substantially decreasing the cost of research.