CORE--TUMOR BIOLOGY

这项计划项目的主要目标是开发新的核酸 杂交技术，基于电荷的先进检测方法 耦合设备，以及自动化样品制备和处理，可以 在自动化工作站中用于分析大的表达 信使核糖核酸种类数、杂合性丧失和特定基因 同时，在单个肺组织样本中发生突变。它是 对记录和量化这些设备的性能至关重要 真实的临床标本。此外，从这些数据中获得的结果 新开发的设备必须与从 传统的方法。肿瘤生物学核心(TBC)负责 协调样本采集，提供预先确定的肿瘤模型 用于标准化的面板和未特定化的肿瘤面板 在项目1和项目3中开发的设备。TBC还将 确定新发现的肺癌基因改变作为来源 为该计划项目增加潜在的有价值的标记。1)至 建立组织库，跟踪所有相关的组织材料 添加到计划项目。2)制备常规组织标本 多参数遗传分析。3)分析基因变化 在肺癌中使用常规手段提供多个标准 参数分析或验证来自多个参数的结果 分析。4)识别额外的基因改变和/或改变 在肺肿瘤发生中可能起重要作用的基因表达 用于更新项目项目中使用的探头面板。

The main goal of this program project is to develop new nucleic acid hybridization technologies, advanced detection methods based on charge couped devices, and automated sample preparation and handling that could be used in an automated workstation to analyze the expression of large numbers of mRNA species, loss of heterozygosity, and specific gene mutations simultaneously, in a single sample of lung tissue. It is crucial to document and quantify the performance of these devices on real clinical specimens. Additionally, the results obtained from these newly developed devices must be comparable to those obtained from conventional methods. Tumor Biology Core (TBC) is responsible for coordinating sample collection, providing precharacterized model tumor panels and uncharacterized tumor panels for the standardization of devices developed in Project 1 and Project 3. The TBC will also identify newly identified genetic alterations n lung cancers as a source of addition potential valuable markers for the Program Project. 1) To establish a tissue bank and keep track of all tissue materials related to the Program Project. 2) To prepare tissue samples for conventional and multi parameter genetic analysis. 3) To analyze genetic alterations in lung cancers using conventional means to provide standards for multi parameter analysis or validate results from the multi parameter analysis. 4) To identify additional genetic alterations and/or changes in mRNA expression which are potentially important in lung tumorigenesis for up-dating probe panels used in the program project.