CORE--IMMUNOLOGY

免疫学核心旨在提供免疫试剂 项目1至4中所需的。这些试剂已建立：单克隆 抗人结直肠癌（CRC）抗原（Ag）的抗体（Mab） GA733和鼠同源物mEGP。这些抗原的多克隆抗体将 在Ag免疫亲和柱上纯化。新单克隆抗体的片段 将生成GA 733 Ag（来自项目1）。抗体从 为病人、健康人和病人接种疫苗（根据项目3） 溃疡性结肠炎，将进行亲和纯化， 结合特异性分析。此外，核心B将产生纯化的 杆状病毒来源的GA 733 Ag和mEGP。一批临床级的 将生产杆状病毒衍生的GA 733 Ag用于患者的皮肤测试 在项目3中。将对所有试剂进行纯度、鉴别和 免疫反应性。 由经验丰富的调查人员提供的集中服务将 提高抗原和抗体制剂的质量， 与将其作为每个项目的一部分进行比较。

The immunology Core is Designed to provide immunologic reagents needed in Project 1 through 4. These reagents are established: monoclonal antibodies (Mabs) to the human colorectal carcinoma (CRC) antigen (Ag) GA733 and the murine homolog mEGP. Polyclonal antibodies to these Ags will be purified on Ag immunoaffinity columns. New Mab to fragments of the GA733 Ag (derived from Project 1) will be generated. Antibodies from vaccinating (under Project 3) patients, healthy individuals and patients with ulcerative colitis, will be affinity-purified for detailed tissue binding specificity analysis. In addition, Core B will produce purified baculovirus-derived GA733 Ag and mEGP. A clinical grade batch of baculovirus-derived GA733 Ag will be produced for skin testing of patients in Project 3. All reagants will be tested for purity, identity, and immunoreactivity. The centralized services provided by experienced investigators will improve quality of the Ag and antibody preparations and save overall costs as compared to their inclusion as part of each project.