RUTHENIUM (II) COMPLEXES AS PROBES OF INTRACELLULAR (INTRA ENDOCYTIC) OXYGEN

我们测量了细胞内外的氧气浓度 用Ru(II)培养小鼠单核-巨噬细胞，J774A.1 具有荧光寿命的配合物[Ru(Bpy)3]2+和[Ru(Phen)3]2+ 显微镜。使用时间分辨技术，我们能够比较 空气和氮气之间对氧气浓度的响应 大气层。相角随氧的变化 胞内外有[Ru(Bpy)3]2+和[Ru(Phen)3]2+。 空气和氮气之间10-12度(400千赫)的数量级。这个 显微镜下的空间分辨率达到了衍射极限， 这使得我们能够测量一个物体内的氧气浓度 单个12微米大小的巨噬细胞。

We measured intra- and extra-cellular concentrations of oxygen in Mouse Monocyte-Macrophage cultures, J774A.1, using the Ru(II) complexes [Ru(bpy)3]2+ and [Ru(phen)3]2+with the fluorescence lifetime microscope. Using the time-resolved technique we were able to compare response for oxygen concentrations between air and nitrogen atmosphere. The oxygen-dependent changes in phase angle for [Ru(bpy)3]2+ and [Ru(phen)3]2+ inside and outside cells were in the order of 10-12 degrees (400 kHz) between the air and nitrogen. The spatial resolution under the microscope was at the diffraction limit, which allowed us to measure the concentration of oxygen inside a single 12 micrometer size macrophage.