CORE--ANIMAL FACILITY

高质量的动物资源计划是DERC基础研究项目的基本组成部分，包括检查胰岛发育和功能，1型和2型糖尿病发病机制，实验治疗，诊断和遗传学。动物核心的主要目标是将四种关键的动物相关资源整合到一个单元中，并为糖尿病相关研究量身定制这些资源。动物：提供与糖尿病研究相关的特定无病原体（SPF）啮齿动物菌株的严格饲养，包括1型和2型实验模型、免疫缺陷菌株、回交/同源系（例如BDC2.5 NOD x rag1(-/-)）和内部生产的转基因和敲除动物。胰岛资源：利用经验丰富的人员从DERC维持的动物中分离啮齿动物胰岛，并在标准分泌/移植方案中提供质量控制分析。转基因设施：在标准（FVB）和定义的近交系（如NOD小鼠）中产生与糖尿病研究相关的新型转基因菌株，并在129SV背景下产生基因敲除和敲入小鼠。基因分型设施：通过PCR/Southern分析监测已建立的和新的突变动物系的遗传完整性，并对杂交动物进行基于微卫星标记的选择，作为“快速遗传”程序的一部分，该程序保留所需等位基因和重要背景基因（例如iddm 1-17）的整合。动物资源中心将部分容纳在现有的芭芭拉戴维斯中心动物设施内，并通过扩展介绍。1135平方英尺的新空间。罗纳德·吉尔博士和戴尔·韦格曼博士将负责指挥中心及其四名人员。

A high-quality animal resources program is a fundamental component of basic research projects within the DERC, including those examining islet development and function, type 1 and 2 diabetes pathogenesis, experimental therapeutics, diagnostic and genetics. The principal objective of the Animal Core is to integrate four key animal-related resources in one unit and tailor these resources to diabetes-related research. The key components are as follows: Animals: To provide stringent husbandry of specific pathogen free (SPF) rodents strains relevant to diabetes research including type 1 and 2 experimental models, immunodeficient strains, backcrossed/congenic lines (e.g. BDC2.5 NOD x rag1(-/-) and transgenic and knockout animals produced in-house. Pancreatic Islet Resources: To isolate rodent islets from DERC- maintained animals using experienced personnel and provide quality control analysis in standard secretion/transplantation protocols. Transgenic facility: To generate novel transgenic strains relevant to diabetes research in standard (FVB) and defined inbred strains (e.g. NOD mice), and to generate gene knockout and knock-in mice on a 129SV background. Genotyping Facility: To monitor the genetic integrity of both established and novel mutant animal lines by PCR/Southern analysis and to perform microsatellite marker-based selection of intercrossed animals as part of a "speed congenic" procedure that preserves the integration of desired alleles and important background genes (e.g. iddm 1-17). The Animal Resources Core will be accommodate in part within the existing Barbara Davis Center animal facility and by expansion intro. 1135 square feet of new space. Dr. Ronald Gill and Dr. Dale Wegmann will direct the Core and its 4 personnel.