SCIENTIFIC CORE

科学核心计划作为成像的中心资源 大鼠脑和三叉神经节切片技术的应用 从豚鼠身上以及从死后的人体组织中提取。 第一个项目将使用受体放射自显影来检测类似5-HT1D的物质 和啮齿动物三叉神经系统中的GABA受体结合部位 死后人脑，以及原位杂交检测 相应的信使核糖核酸。 第二个项目将使用[14C]2-脱氧葡萄糖放射自显影技术 测量局部葡萄糖利用作为神经细胞的指标 痛苦的硬脑膜刺激后大鼠脑内的激活。神经元 同样的动物(在连续的大脑中)的激活也将被评估 部分)通过检测早期基因c-fos的信使RNA 原位杂交。使用相同的刺激方案，我们将使用 [14C]安替比林碘放射自显影在另一组动物中的测定 脑膜伤害性刺激对代谢/血流量的影响 耦合。 这些实验的结果可能会影响对 将功能激活研究作为最后一个项目的目标。

The Scientific Core is intended to serve as a central resource for imaging techniques applied to brain and trigeminal ganglion sections from rats and guinea pigs, as well as from post mortem human tissue. The first project will use receptor autoradiography to detect 5-HT 1D-like and GABA receptor binding sites ina the trigeminal system of rodents and post mortem human brain, as well as in situ hybridization to detect the corresponding messenger RNA's. The second project will use [14C]2-deoxyglucose autoradiography technique to measure regional glucose utilization as an indicator of neuronal activation in the rat brain after painful dural stimulation. Neuronal activation will also be assessed in the same animals (in consecutive brain sections) by detecting the messenger RNA of the early gene c-fos using in situ hybridization. Using the same stimulus, we will use [14C]iodoantipyrine autoradiography in another set of animals to determine the consequences of noxious meningeal stimulation on metabolism/blood flow coupling. The results of such experiments may impact on the interpretation of functional activation studies as an aim of the last project.