Determining how Aly links splicing to mRNA export

这一建议的长期目标是实现对前mRNA剪接和mRNA输出之间的耦合的详细了解。蛋白质Aly在剪接体组装过程中被招募到mRNA中，并特异性地促进剪接mRNA的输出，可能在连接剪接和mRNA输出中发挥关键作用。因此，详细了解该蛋白及其与这两个主要核机制的相互作用对于理解基因表达是至关重要的。第一个具体目标是定位Aly中与剪接体和出口机制相互作用所需的功能结构域。第二个特定目的是确定在剪接体组装过程中Aly是如何被招募到mRNA中的。最后，第三个具体目标是确定如何以出口机制为目标。总之，所提出的研究将导致对剪接和信使核糖核酸输出之间的耦合以及信使核糖核酸输出机制的重要见解。

The long-term objective of this proposal is to achieve a detailed understanding of the coupling between pre-mRNA splicing and mRNA export. The protein Aly, which is recruited to mRNA during spliceosome assembly and specifically promotes the export of spliced mRNA, is likely to play a key role in linking splicing to mRNA export. Thus, a detailed understanding of this protein and its interactions with these two major nuclear machineries is essential for understanding gene expression. The first specific aim is to map the functional domains in Aly that are required for interactions with the spliceosome and the export machinery. The second specific aim is to determine how Aly is recruited to the mRNA during spliceosome assembly. Finally, the third specific aim is to determine how Aly is targeted to the export machinery. Together, the proposed studies should lead to important insights into the coupling between splicing and mRNA export as well as into the mechanism of mRNA export.