DOUBLE-STRANDED RNA CONDENSATION BY MULTIVALENT CATION

描述该项目的目的是阐明双链（ds）RNA缩合的机制。dsRNA是基因组包装中的生物学机制，由于其在非病毒基因递送领域的重要性，已受到大量关注。然而，尽管基因组dsRNA在某些病毒中的包装或通常发现的单链RNA中的dsRNA区域与蛋白质的关联，但dsRNA缩合完全缺乏关注。这种电子显微镜的主要目标将被用来遵循凝聚动力学和聚集体形态。还将使用通常用于缩合dsDNA的阳离子配体研究配体结构和迁移率对dsRNA缩合的影响。介电常数对dsRNA缩合的影响将使用水-醇混合物来解决，以确定螺旋电荷密度和刚度的作用。该项目的最终目标是对相同轮廓的dsDNA和dsRNA片段进行明确比较。

DESCRIPTION The objective of this proposed project is the elucidation of the mechanism of double-stranded (ds) RNA condensation dsRNA, the biological mechanism in genome packaging, has received a significant amount of attention because of its importance in the field of non-viral gene delivery. Yet, dsRNA condensation has received a complete lack of attention has a complete lack of attention despite the packaging of genomic dsRNA in certain viruses or associations of dsRNA regions in commonly found single-stranded RNA with proteins. A primary goal of this electron microscopy will be used to follow the condensation kinetics and aggregate morphologies. The effects of ligand structure and mobility on dsRNA condensation will also be studied using cartionic ligands commonly used to condense dsDNA. The effect of dielectric constant on dsRNA condensation will be addressed using water-alcohol mixtures to determine the roles of helical charge density and stiffness. The final goal of this project is a definitive comparison of dsDNA and dsRNA fragment of equal contour.