PATHOGENESIS OF NON BACTERIAL CHRONIC PROSTATITIS

淋巴细胞和淋巴细胞衍生的细胞因子被认为通过调节包括炎性细胞、免疫细胞和组织细胞（例如肥大细胞）在内的多种细胞的功能而在控制慢性炎症过程中至关重要。 目前，对T淋巴细胞、肥大细胞、神经及其产物在非细菌性慢性前列腺炎（NBCP）及其相关慢性疼痛中的存在和作用知之甚少。基于越来越多的关于淋巴细胞-肥大细胞-神经相互作用在疼痛中的作用的文献，我们提出了以下假设：假设：非细菌性慢性前列腺炎（NBCP）、前列腺痛和慢性非细菌性炎症（CNBI）是由于特定T淋巴细胞亚群失衡引起的前列腺炎性疾病（T1对T2），其通过其细胞因子表达直接和间接调节前列腺微环境内的炎症、免疫和组织细胞（肥大细胞和神经）的功能，从而控制慢性发炎前列腺组织内的炎症和疼痛。 为了检验这一假设，我们提出了以下目标：1）分离、表征和关联来自NBCP、前列腺痛、CNBI和对照患者群体的流体（血液、尿液、前列腺液）和组织中的T细胞。2)表征NBCP、前列腺痛、CNBI和对照患者的液体和组织中T1和T2细胞因子的分布并建立相关性。3)表征NBCP、前列腺痛、CNBI和对照患者中肥大细胞和肥大细胞相关因子的分布并使其相关。4)明确NBCP、前列腺痛、CNBI和对照组中淋巴细胞、肥大细胞和神经之间的关系。5)使用已建立的经验证的仪器量化慢性前列腺炎（NBCP、前列腺痛和CNBI）患者的疼痛，以将其临床病程与细胞和细胞因子结果相关联。

Lymphocytes and lymphocyte-derived cytokines are thought to be critical in controlling chronic inflammatory processes by regulating the function of a wide variety of cells including inflammatory, immunologic and tissue cells (e.g. mast cells). Currently, little is known about the existence and role of T lymphocytes, mast cells, nerves and their products in non-bacterial chronic prostatitis (NBCP) and its associated chronic pain. Based on the growing body of literature on the role of lymphocyte-mast cell-nerve interactions in pain we have developed the following hypothesis: Hypothesis: Non-bacterial chronic prostatitis (NBCP), prostatodynia and chronic nonbacterial inflammation (CNBI) are inflammatory diseases of the prostate that result from an imbalance of specific T lymphocyte subpopulations (T1 vs. T2), which via their cytokine expression, regulate directly and indirectly the function of inflammatory, immunology and tissue cells (mast cells and nerves) within the prostate microenvironment, and thereby control both inflammation and pain within chronically inflamed prostate tissue. To test this hypothesis we propose the following goals: 1) to isolate, characterize and correlate T cells in fluids (blood, urine, prostate fluids) and tissue from NBCP, prostatodynia, CNBI and control patient populations. 2) to characterize and correlate the distribution of T1 and T2 cytokines in fluids and tissue from NBCP, prostatodynia, CNBI and control patients. 3) to characterize and correlate the distribution of mast cells and mast cell-related factors in NBCP, prostatodynia, CNBI and control patients. 4) to define the relationships between lymphocytes, mast cells and nerves in NBCP, prostatodynia, CNBI and control patients. 5) to quantify pain in patients with chronic prostatitis (NBCP, prostatodynia, and CNBI) using established validated instruments to correlate their clinical course with cell and cytokine results.