ION CHANNELS AND PULMONARY VASCULAR TONE

描述(改编自申请人的摘要)：这是一项建议 肺血管钾通道的生理学作用 使它们对低氧的反应更加平稳。核心假设是两者都 急性和慢性缺氧在功能或转录水平上抑制钾通道 级别分别为。这些通道的抑制会导致Em(膜 电压门控钙通道启动的去极化和开放 缺氧性血管收缩。其目的是：1)电生理学特性 K通道的性质和确定天然K(V)的分子基础 肺动脉平滑肌细胞(PASMC)；2)鉴定Kv通道α 决定全细胞Ik(V)和调节Em的亚基；3)指定Kv 对低氧敏感并确定低氧机制的通道 介导性抑制；4)检测慢性低氧对机体功能和功能的影响。 PASMC中Kv通道的表达。

DESCRIPTION (Adapted from the Applicant's Abstract): This is a proposal to examine the physiologic contributions of K channels in pulmonary vascular smooth towards their response to hypoxia. The central hypotheses are that both acute and chronic hypoxia inhibit K channels at a functional or transcriptional level, respectively. Inhibition of these channels results in Em (membrane potential) depolarization and opening of voltage gated Ca channels initiating hypoxic vasoconstriction. The aims are: 1) characterize electrophysiological properties of K channels and determine the molecular basis of native IK(V) in pulmonary artery smooth muscle cells (PASMC); 2) identify Kv channel alpha subunits that determine whole cell IK(V) and regulate Em; 3) specify Kv channels that are sensitive to hypoxia and define the mechanism of hypoxic mediated inhibition; and 4) examine effects of chronic hypoxia on function and expression of Kv channels in PASMC.