Intracellular survival determinants of Yersinia pestis

描述(申请人提供)：鼠疫耶尔森氏菌是鼠疫的病原体， 一种急性的，通常是致命的细菌感染，通过跳蚤叮咬或 气雾剂。鼠疫耶尔森氏菌被认为是一种兼性胞内致病菌。 先前的数据表明，鼠疫杆菌在体内存活并复制 巨噬细胞在感染的前几个小时，而细胞外 在后来的时间点上，增长是主导的。鼠疫杆菌的致病机制 在巨噬细胞中存活和复制的情况尚不清楚。长期目标 这项拟议的研究的目的是确定 鼠疫杆菌在巨噬细胞中的细胞内存活。为了实现这一目标，我们 将(1)描述巨噬细胞防御对于限制 鼠疫杆菌在细胞内的存活和复制；以及(2)鉴定 鼠疫耶尔森氏菌对对抗鼠疫杆菌的胞内防御很重要 巨噬细胞。了解鼠疫杆菌的胞内生存机制 可以帮助开发预防或治疗鼠疫感染的新方法 人类人口。

DESCRIPTION (provided by applicant): Yersinia pestis is the agent of plague, an acute, often fatal bacterial infection that is transmitted by flea bite or aerosol. Y. pestis is considered to be a facultative intracellular pathogen. Previous data indicate that Y. pestis survives and replicates within macrophages during the first few hours of infection, while extracellular growth is predominant at later time points. The mechanism by which Y. pestis survives and replicates in macrophages is not known. The long-term objective of the proposed studies is to determine the molecular and cellular basis for intracellular survival of Y. pestis in macrophages. To achieve this goal we will (1) Characterize macrophage defenses that are important for limiting intracellular survival and replication of Y. pestis; and (2) Identify genes in Y. pestis that are important for combating intracellular defenses of macrophages. Understanding the intracellular survival mechanism of Y. pestis may aid the development of new ways to prevent or treat plague infections in the human population.