GALLBLADDER MUSCARINIC RECEPTORS IN ACUTE CHOLECYSTITIS

描述：胆囊功能障碍是重要的健康问题， 由于在急性和慢性胆囊炎患者中均观察到明显的胆囊运动功能障碍， 胆囊炎该建议将利用胆总管结扎术， 豚鼠（作为急性非结石性胆囊炎模型），以检查 假设局部炎症介质的作用，在早期 胆囊炎对胆囊M受体亚型表达的影响 下游信号转导机制负责胆囊 收缩。本建议的初步重点是确定 毒蕈碱受体亚型及其信号转导通路对正常人的影响 胆囊收缩这项建议的第二个重点是， 描述急性炎症和炎症介质的作用 （类花生酸，细胞因子）对胆囊毒蕈碱受体亚型的影响， 外部信号的传导。这一建议将提供深入了解这两个 急性胆囊炎的病理生理学和一般机制 在急性胆囊炎发作期间降低胆囊收缩力 炎症拟议研究的结果将确定未来 预防胆囊运动功能障碍的方法研究 炎症

DESCRIPTION: Disorders of gallbladder function are important health concerns, since marked gallbladder motor dysfunction is seen in both acute and chronic cholecystitis. This proposal will utilize common bile duct ligation in the guinea pig (as a model of acute acalculous cholecystitis) to examine the hypothesis that the effects of local inflammatory mediators during early cholecystitis alter gallbladder muscarinic receptor subtype expression and the downstream signal transduction mechanisms responsible for gallbladder contraction. The initial focus of this proposal is to determine the role of muscarinic receptor subtypes and their signal transduction pathways on normal gallbladder contraction. The second major focus of this proposal is to delineate the effects of acute inflammation and inflammatory mediators (eicosanoids, cytokines) on gallbladder muscarinic receptor subtypes and the transduction of external signals. This proposal will provide insight into both the pathophysiology of acute cholecystitis and the general mechanisms involved in decreasing gallbladder contractility during initiation of acute gallbladder inflammation. The results from the proposed studies will identify future studies on ways to prevent the motor dysfunction that occurs with gallbladder inflammation.