Defining the role of Death Associated Protein Kinase 1 in cell fate using genomic and proteomic analysis

项目概述：死亡相关蛋白激酶1（DAPK1）是人类细胞命运的关键调节因子，作为自噬细胞死亡的控制点，并与癌症和神经变性有关。本项目的目的是利用基因组学和蛋白质组学技术的新进展，结合对DAPK 1酶功能的新认识，来剖析这种蛋白质下游的通路。这将反过来帮助定义这种重要蛋白质的细胞作用，并帮助开发新的药物来调节DAPK1。这四年的学生，由BBSRC的工业案例奖资助，将部分基于阅读药学院的大学，部分基于生物信息学领域的领导者BC平台。学生将使用细胞和生物化学方法来研究DAPK1，从而对调节酶活性的细胞影响进行基因组和蛋白质组学分析。后者的生物信息学调查将通过BC平台进行，提供行业经验。在博士课程期间，学生将有机会在阅读大学的亨利商学院注册工商管理证书。

Project Overview: Death associated protein kinase 1 (DAPK1) is key regulator of cell fate in human cells, acting as a control point for autophagic cell death, and has been implicated in cancer and neurodegneration. Despite two decades of research, the molecular pathways coordinated by DAPK1 remain poorly defined.The aim of this project is to use new advances in genomic and proteomic technology, coupled with novel insights into the enzymatic function of DAPK1, to dissect the pathways downstream of this protein. This will, in turn, help define the cellular role of this important protein and aid in the development of new drugs to modulate DAPK1.This four year studentship, funded by an Industrial CASE award from the BBSRC, will be based partly at the University of Reading School of Pharmacy and partly at BC platforms, a leader in the field of bioinformatics. The student will use cellular and biochemical methods to characterise DAPK1, leading on to genomic and proteomic analysis of the cellular impact of modulating the enzymes activity. Bioinformatic investigation of the latter will be carried out with BC platforms, providing industry experience. During the course of the PhD, the student will have the opportunity to register for a Certificate in Business Administration at Henley Business School, part of the University of Reading.