AXON GUIDANCE IN THE DROSOPHILA VISUAL SYSTEM

这是香农奖，为这项研究提供部分支持 项目低于指定研究所的资助范围，但 都在最优秀的边缘。香农奖旨在提供 支持测试该方法的可行性；开发进一步的测试 并改进研究方法；进行二次分析或可用 数据集；或执行可演示PI的离散项目 研究能力或为已经功成名就的 申请。下面的摘要摘自原始文件 由首席调查员提交。 描述(改编自申请人的摘要)： 拟议的研究是寻找轴突生长所需的基因， 使用突变方法的寻路和目标识别。这个 动物模型是为果蝇幼虫的视神经提供 这是上述现象的一个简单模型。视神经跟随 细胞连接桥上的固定路径，有两个区域 生长锥体暂停，然后进行方向性改变。使用 化学诱变筛选已分离到的一批突变体 影响视神经在其路径上的不同点。这个 其中一种名为 摘要(Abs)构成了提案的核心。随诊研究 将(1)试图描述abs的特征以获得洞察力。 对其生化功能进行分析；(2)分析其丢失的表型。 胚胎和成像期功能突变的功能或获得 形成圆盘，希望揭示其功能；以及(3)进行 分析abs与其他基因的相互作用以确定其 在更复杂的蛋白质变化背景中发挥作用。

THIS IS A SHANNON AWARD PROVIDING PARTIAL SUPPORT FOR THE RESEARCH PROJECTS THAT FALL SHORT OF THE ASSIGNED INSTITUTE'S FUNDING RANGE BUT ARE IN THE MARGIN OF EXCELLENCE. THE SHANNON AWARD IS INTENDED TO PROVIDE SUPPORT TO TEST THE FEASIBILITY OF THE APPROACH; DEVELOP FURTHER TESTS AND REFINE RESEARCH TECHNIQUES; PERFORM SECONDARY ANALYSIS OR AVAILABLE DATA SETS; OR CONDUCT DISCRETE PROJECTS THAT CAN DEMONSTRATE THE PI'S RESEARCH CAPABILITIES OR LEND ADDITIONAL WEIGHT TO AN ALREADY MERITORIOUS APPLICATION. THE ABSTRACT BELOW IS TAKEN FROM THE ORIGINAL DOCUMENT SUBMITTED BY THE PRINCIPAL INVESTIGATOR. DESCRIPTION (Adapted from applicant's abstract): The goal of the proposed research is to find genes required for axonal growth, pathfinding and target recognition using a mutagenetic approach. The animal model is the Drosophila optic nerve of the larva which provides a simple model of the aforementioned phenomena. The optic nerve follows a stereotyped path over a connecting bridge of cells and has two regions where growth cones pause and then make directional changes. Using a chemical mutagenesis screen a number of mutants have been isolated that effect the optic nerve at various points along its pathway. The molecular and genetic characterization of one of the mutations called abstract (abs) forms the heart of the proposal. The follow up studies that are proposed will (1) attempt to characterize abs to gain insights into its biochemical function; (2) analyze the phenotype of loss of function or gain of function mutations in the embryo and during imaginal disc formation in hopes of revealing its function; and (3) undertake a genetic analysis of abs as it interacts with other genes to define its role within a more complex background of altered proteins.