MULTI-PHOTON CONFOCAL MICROSCOPE

该申请的目的是用最先进的多光子共聚焦显微镜升级堪萨斯大学现有的核心共聚焦设备。在过去的五年中，超过40名研究人员使用了核心共聚焦设备进行研究。此时，共聚焦显微镜已经过时，有失效的危险。它无法升级，主要是因为制造商Meridian倒闭了。因此，本提案的目标是为核心共聚焦设施购买多光子共聚焦显微镜。新的多光子显微镜将取代现有的子午线共聚焦显微镜。该显微镜将提供给堪萨斯大学系统内的所有研究人员，以及其他机构的研究人员。然而，在多光子显微镜上完成的大部分研究项目将来自9个实验室。这些调查人员已被确定为新仪器的“主要用户”。他们的研究领域涵盖了几个主题，简要介绍。Drs。南希·伯曼和保罗·切尼探讨了艾滋病毒对脑组织的影响。他们将使用多光子显微镜对标记为神经元标记的大脑切片进行成像。S.K. Dey博士研究了小鼠胚胎的植入和围绕这一事件激活的信号通路。他用共聚焦显微镜观察信号事件和免疫染色组织。Jared Grantham博士使用免疫染色和共聚焦成像技术研究肾脏疾病中MAP激酶途径的一个组成部分。Michael Sarras博士主要研究使用双波长荧光共聚焦显微镜的细胞/细胞外基质相互作用。Lisa Stehno-Bittel博士利用膜片钳和共聚焦成像技术研究分子在核膜上的转移。Michael Werele博士使用共聚焦显微镜检查控制突触形成、稳定和消除的机制。Doug Wright博士利用抗体和轴突跟踪器共聚焦成像研究损伤和疾病中的外周感觉神经元。在医学中心校园增加多光子共聚焦显微镜将极大地加强这些美国国立卫生研究院资助的研究人员以及该地区其他教职员工的研究工作。

The purpose of the application is to upgrade the existing Core Confocal Facility at the University of Kansas with a state-of-the-art multi-photon confocal microscope. Over forty investigators have used the Core Confocal Facility for their research for the past five years. At this time the confocal microscope is dated and at risk of failure. It can not be upgraded mainly because the manufacturer, Meridian, is out of business. Thus, the goal of this proposal is to purchase a multi-photon confocal microscope for the Core Confocal Facility. The new multi-photon microscope would replace the existing Meridian confocal scope. The microscope would be available to all investigators within the University of Kansas system, and to researchers from other institutions. However, the majority of the research projects completed on the multi-photon microscope would come from nine laboratories. These investigators have been identified as "major users" of the new instrument. Their research areas cover several topics, which are described briefly. Drs. Nancy Berman and Paul Cheney explore the effects of HIV on brain tissue. They will use the multi-photon microscope to image brain slices labeled for neuronal markers. Dr, S.K. Dey studies implantation of mouse embryos and the signaling pathways activated around that event. He used a confocal microscope for visualizing signaling events and immunostaining tissue. Dr. Jared Grantham studies a component of the MAP kinase pathway in renal disease using immunostaining and confocal imaging. Dr. Michael Sarras focuses his research on cell/extracellular matrix interactions using dual wavelength fluorescence confocal microscopy. Dr. Lisa Stehno-Bittel investigates the transfer of molecules across the nuclear membrane using patch-clamp and confocal imaging. Dr. Michael Werele uses confocal microscopy to examine the mechanisms controlling synapse formation, stabilization, and elimination. Dr. Doug Wright studies the peripheral sensory neurons in injury and disease using antibodies and axon trackers for confocal imaging. The addition of a multi-photon confocal microscope to the Medical Center campus will greatly enhance the research efforts of these NIH-funded investigators along with other faculty members in the area.