ABI PRISM 377 DNA SEQUENCER

我们建议的目的是在内科建立基因组学核心设施，以进行高通量基因分型和基于DNA基因组学的临床研究，并促进该系其他研究人员合作研究复杂疾病的遗传基础。此外，核心设施将用于初级临床研究人员通过大学临床研究中心提出的试点项目。我们正在申请资金购买ABI Prism 377 DNA测序仪用于这个基因组核心设施。目前，内科有一台ABI Prism 310 DNA基因分析仪，该系的研究人员正在全天候使用。这台机器的用户主要是私家侦探和合作调查人员，他们平均每月运行1400次，主要用于基因型分析。由于机器的密集使用，分析样品的平均等待时间为4周。ABI 310是德克萨斯大学休斯顿医学院唯一可用于基因型分析的测序仪。根据合作研究人员目前资助的项目的拟议目标，预计使用量会增加。ABI Prism 377测序仪10%的时间将保留给通过大学临床研究中心开展涉及基因组学临床研究的初级研究人员。因此，ABI Prism 377 DNA测序仪的加入将显著增强德克萨斯大学休斯顿医学院正在进行的和未来的基于基因组学的临床研究。

The aim of our proposal is to establish a Genomics Core Facility for high throughput genotyping and DNA genomics-based clinical research in the Department of Internal Medicine and foster collaborations to study the genetic basis of complex diseases by other investigators in the Department. In addition, the core facility will be used for pilot projects proposed by junior clinical investigators through the University Clinical Research Center. We are applying for funds to purchase an ABI Prism 377 DNA Sequencer for this Genomic Core Facility. Currently the Department of Internal Medicine has one ABI Prism 310 DNA Genetic Analyzer, which is being used around the clock by researchers within the Department. The users of this machine are primarily the P.I. and collaborating investigators, and they average 1400 runs per month, primarily for genotype analysis. The average waiting period to analyze samples is 4 weeks because of the intensive use of the machine. The ABI 310 is the only sequencer available for genotype analysis within The University of Texas-Houston Medical School. Increased usage is projected based on the proposed aims of currently funded projects of the collaborating investigators. Ten percent of the time the ABI Prism 377 Sequencer will be reserved for junior investigators initiating pilot studies involving genomics-based clinical research through the University Clinical Research Center. Therefore, addition of the ABI Prism 377 DNA Sequencer would significantly enhance ongoing and future genomics- based clinical research within the University of Texas-Houston Medical School.