Selenium Enriched Plant Material for Chemoprevention

描述（从申请人的摘要扫描）：硒可以发挥 在癌症预防中的重要作用。膳食硒有助于预防 几种类型的癌，包括肺癌、前列腺癌和结肠癌。的效力 硒因其化学形式而异。最有效的化合物是迅速和 定量转化的身体，如硒-甲基硒代半胱氨酸，其中之一， 最有效的抗癌硒化合物。不贵的香油 提供膳食硒的方法是使某些食品富含硒， Se-甲基硒代半胱氨酸的形式。我们建议制造这种产品 通过工程作物积累硒-甲基硒代半胱氨酸。一期 研究已经确定了改变硒代谢的可行性， 拟南芥通过过表达ATP硫酸化酶，导致更多的 有效吸收硒酸盐。硒代半胱氨酸甲基转移酶。 bisulcatus在A. thaliana.进一步改善 这些植物吸收和转化硒酸盐的能力涉及克隆 参与硒积累的其他基因。根据第一阶段的结果， 研究，基因参与硒吸收的根和化学还原 将靶向组织中的硒酸盐至亚硒酸盐。我们会把这些 基因注入印度芥菜， 商业上可行的高Se-甲基硒代半胱氨酸植物产品。 拟议商业应用：不可用

DESCRIPTION (Scanned from the Applicant's Abstract): Selenium may play a significant role in cancer prevention. Dietary selenium aids in preventing several types of carcinomas including lung, prostate, and colon. The potency of Se varies with its chemical form. The most effective compounds are rapidly and quantitatively transformed by the body, such as Se-methylselenocysteine, one of the most effective anti-carcinogenic Se-compounds. An inexpensive way to provide dietary Se would be to enrich certain foodstuffs with Se, preferably in the form of Se-methylselenocysteine. We propose to create this type of product by engineering crop plants to accumulate Se -methylselenocysteine. Phase I studies have established the feasibility of altering selenium metabolism in Arabidopsis thaliana by overexpressing ATP sulfurylase, resulting in more efficient assimilation of selenate. Selenocysteine methyltransferase from A. bisulcatus was also expressed in A. thaliana. Further improvements in the ability of these plants to take up and transform selenate involve cloning additional genes involved in Se accumulation. Based on results of the Phase I research, genes involved in Se uptake in the roots and chemical reduction of selenate to selenite in the tissues will be targeted. We will transfer these genes into Brassica juncea, the Indian mustard plants, to produce a commercially viable high Se-methylselenocysteine plant product. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE